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Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies.

Publication ,  Journal Article
Harris, SL; Craig, L; Mehroke, JS; Rashed, M; Zwick, MB; Kenar, K; Toone, EJ; Greenspan, N; Auzanneau, FI; Marino-Albernas, JR; Pinto, BM; Scott, JK
Published in: Proceedings of the National Academy of Sciences of the United States of America
March 1997

To investigate the molecular basis of antigenic mimicry by peptides, we studied a panel of closely related mAbs directed against the cell-wall polysaccharide of group A Streptococcus. These antibodies have restricted V-gene usage, indicating a shared mechanism of binding to a single epitope. Epitope mapping studies using synthetic fragments of the cell-wall polysaccharide supported this conclusion. All of the mAbs isolated crossreactive peptides from a panel of phage-displayed libraries, and competition studies indicated that many of the peptides bind at or near the carbohydrate binding site. Surprisingly, the peptides isolated by each mAb fell into distinct consensus-sequence groups that discriminated between the mAbs, and in general, the peptides bound only to the mAbs used for their isolation. Similar results were obtained with polyclonal antibodies directed against synthetic oligosaccharide fragments of the streptococcal cell-wall polysaccharide. Thus, the peptides appear to be specific for their isolating antibodies and are not recognized by the same mechanism as their carbohydrate counterparts.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

March 1997

Volume

94

Issue

6

Start / End Page

2454 / 2459

Related Subject Headings

  • Streptococcus pyogenes
  • Shigella flexneri
  • Salmonella
  • Polysaccharides, Bacterial
  • Peptides
  • Oligosaccharides
  • Oligopeptides
  • Molecular Sequence Data
  • Immunoglobulin kappa-Chains
  • Immunoglobulin M
 

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Harris, S. L., Craig, L., Mehroke, J. S., Rashed, M., Zwick, M. B., Kenar, K., … Scott, J. K. (1997). Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies. Proceedings of the National Academy of Sciences of the United States of America, 94(6), 2454–2459. https://doi.org/10.1073/pnas.94.6.2454
Harris, S. L., L. Craig, J. S. Mehroke, M. Rashed, M. B. Zwick, K. Kenar, E. J. Toone, et al. “Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies.Proceedings of the National Academy of Sciences of the United States of America 94, no. 6 (March 1997): 2454–59. https://doi.org/10.1073/pnas.94.6.2454.
Harris SL, Craig L, Mehroke JS, Rashed M, Zwick MB, Kenar K, et al. Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies. Proceedings of the National Academy of Sciences of the United States of America. 1997 Mar;94(6):2454–9.
Harris, S. L., et al. “Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies.Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 6, Mar. 1997, pp. 2454–59. Epmc, doi:10.1073/pnas.94.6.2454.
Harris SL, Craig L, Mehroke JS, Rashed M, Zwick MB, Kenar K, Toone EJ, Greenspan N, Auzanneau FI, Marino-Albernas JR, Pinto BM, Scott JK. Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies. Proceedings of the National Academy of Sciences of the United States of America. 1997 Mar;94(6):2454–2459.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

March 1997

Volume

94

Issue

6

Start / End Page

2454 / 2459

Related Subject Headings

  • Streptococcus pyogenes
  • Shigella flexneri
  • Salmonella
  • Polysaccharides, Bacterial
  • Peptides
  • Oligosaccharides
  • Oligopeptides
  • Molecular Sequence Data
  • Immunoglobulin kappa-Chains
  • Immunoglobulin M