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Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase.

Publication ,  Journal Article
Dobrikov, MI; Sergueeva, ZA; Shaw, BR
Published in: Nucleosides, nucleotides & nucleic acids
May 2003
Published version (DOI)

The Rp-stereoisomer of 5'-(alpha-P-borano)triphosphates of 2'-deoxycytidine (Rp-dCTPalphaB) and 2',3'-dideoxycytidine (Rp-ddCTPalphaB) were synthesized. Their steady-state kinetics of incorporation by ddNTP-resistant enzymes, e.g., MMLV reverse transcriptase (RT) and Taq DNA polymerase, were investigated and compared with incorporation of dCTP and ddCTP. The alpha-boranophosphate substitution in ddCTP results in a 28-fold increase in efficiency of incorporation of the Rp-ddCTPalphaB isomer by MMLV RT, yet has minimal effect on the efficiency of incorporation by Taq DNA polymerase.

Duke Scholars

Barbara R. Shaw
Author Barbara R. Shaw Chemistry
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Published In

Nucleosides, nucleotides & nucleic acids

DOI

10.1081/ncn-120023091

EISSN

1532-2335

ISSN

1525-7770

Publication Date

May 2003

Volume

22

Issue

5-8

Start / End Page

1651 / 1655

Related Subject Headings

  • Taq Polymerase
  • RNA-Directed DNA Polymerase
  • Organic Chemistry
  • Moloney murine leukemia virus
  • Mice
  • Kinetics
  • Dideoxynucleotides
  • Deoxycytosine Nucleotides
  • Cytidine Triphosphate
  • Animals
 

Citation

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ICMJE
MLA
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Dobrikov, M. I., Sergueeva, Z. A., & Shaw, B. R. (2003). Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase. Nucleosides, Nucleotides & Nucleic Acids, 22(5–8), 1651–1655. https://doi.org/10.1081/ncn-120023091
Dobrikov, Mikhail I., Zinaida A. Sergueeva, and Barbara Ramsay Shaw. “Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase.” Nucleosides, Nucleotides & Nucleic Acids 22, no. 5–8 (May 2003): 1651–55. https://doi.org/10.1081/ncn-120023091.
Dobrikov MI, Sergueeva ZA, Shaw BR. Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase. Nucleosides, nucleotides & nucleic acids. 2003 May;22(5–8):1651–5.
Dobrikov, Mikhail I., et al. “Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase.” Nucleosides, Nucleotides & Nucleic Acids, vol. 22, no. 5–8, May 2003, pp. 1651–55. Epmc, doi:10.1081/ncn-120023091.
Dobrikov MI, Sergueeva ZA, Shaw BR. Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase. Nucleosides, nucleotides & nucleic acids. 2003 May;22(5–8):1651–1655.

Published In

Nucleosides, nucleotides & nucleic acids

DOI

10.1081/ncn-120023091

EISSN

1532-2335

ISSN

1525-7770

Publication Date

May 2003

Volume

22

Issue

5-8

Start / End Page

1651 / 1655

Related Subject Headings

  • Taq Polymerase
  • RNA-Directed DNA Polymerase
  • Organic Chemistry
  • Moloney murine leukemia virus
  • Mice
  • Kinetics
  • Dideoxynucleotides
  • Deoxycytosine Nucleotides
  • Cytidine Triphosphate
  • Animals