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Fe3+ coordination and redox properties of a bacterial transferrin.

Publication ,  Journal Article
Taboy, CH; Vaughan, KG; Mietzner, TA; Aisen, P; Crumbliss, AL
Published in: The Journal of biological chemistry
January 2001

The Fe(3+) binding site of recombinant nFbp, a ferric-binding protein found in the periplasmic space of pathogenic Neisseria, has been characterized by physicochemical techniques. An effective Fe(3+) binding constant in the presence of 350 microm phosphate at pH 6.5 and 25 degrees C was determined as 2.4 x 10(18) m(-1). EPR spectra for the recombinant Fe(3+)nFbp gave g' = 4.3 and 9 signals characteristic of high spin Fe(3+) in a strong ligand field of low (orthorhombic) symmetry. (31)P NMR experiments demonstrated the presence of bound phosphate in the holo form of nFbp and showed that phosphate can be dialyzed away in the absence of Fe(3+) in apo-nFbp. Finally, an uncorrected Fe(3+/2+) redox potential for Fe-nFbp was determined to be -290 mV (NHE) at pH 6.5, 20 degrees C. Whereas our findings show that nFbp and mammalian transferrin have similar Fe(3+) binding constants and EPR spectra, they differ greatly in their redox potentials. This has implications for the mechanism of Fe transport across the periplasmic space of Gram-negative bacteria.

Duke Scholars

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

January 2001

Volume

276

Issue

4

Start / End Page

2719 / 2724

Related Subject Headings

  • Transferrin
  • Nuclear Magnetic Resonance, Biomolecular
  • Neisseria
  • Models, Molecular
  • Models, Chemical
  • Iron
  • Ferric Compounds
  • Electron Spin Resonance Spectroscopy
  • Biological Transport
  • Biochemistry & Molecular Biology
 

Citation

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Taboy, C. H., Vaughan, K. G., Mietzner, T. A., Aisen, P., & Crumbliss, A. L. (2001). Fe3+ coordination and redox properties of a bacterial transferrin. The Journal of Biological Chemistry, 276(4), 2719–2724. https://doi.org/10.1074/jbc.m004763200
Taboy, C. H., K. G. Vaughan, T. A. Mietzner, P. Aisen, and A. L. Crumbliss. “Fe3+ coordination and redox properties of a bacterial transferrin.The Journal of Biological Chemistry 276, no. 4 (January 2001): 2719–24. https://doi.org/10.1074/jbc.m004763200.
Taboy CH, Vaughan KG, Mietzner TA, Aisen P, Crumbliss AL. Fe3+ coordination and redox properties of a bacterial transferrin. The Journal of biological chemistry. 2001 Jan;276(4):2719–24.
Taboy, C. H., et al. “Fe3+ coordination and redox properties of a bacterial transferrin.The Journal of Biological Chemistry, vol. 276, no. 4, Jan. 2001, pp. 2719–24. Epmc, doi:10.1074/jbc.m004763200.
Taboy CH, Vaughan KG, Mietzner TA, Aisen P, Crumbliss AL. Fe3+ coordination and redox properties of a bacterial transferrin. The Journal of biological chemistry. 2001 Jan;276(4):2719–2724.

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

January 2001

Volume

276

Issue

4

Start / End Page

2719 / 2724

Related Subject Headings

  • Transferrin
  • Nuclear Magnetic Resonance, Biomolecular
  • Neisseria
  • Models, Molecular
  • Models, Chemical
  • Iron
  • Ferric Compounds
  • Electron Spin Resonance Spectroscopy
  • Biological Transport
  • Biochemistry & Molecular Biology