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Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM.

Publication ,  Journal Article
Morell, G; Steplock, D; Shenolikar, S; Weinman, EJ
Published in: Am J Physiol
December 1990

Previous in vitro studies with detergent-solubilized rabbit renal brush-border membrane (BBM) proteins have suggested that adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase A (PKA)-mediated inhibition of the Na(+)-H+ exchanger requires the presence of 42-kDa cofactor that is distinct from the exchanger itself. We sought to determine whether there was a protein in native rabbit renal BBM vesicles that has characteristics similar to that of the 42-kDa cofactor. Incubation of native BBM vesicle proteins with a hypotonic phosphorylation solution containing purified catalytic subunit of PKA resulted in phosphorylation of a number of BBM proteins, including a protein with an apparent molecular weight that was similar but not identical to that of the 42-kDa cofactor obtained from anion-exchange column chromatography of n-octyl glucoside-extracted BBM proteins. The identity between the BBM vesicle protein and the 42-kDa cofactor was established by phosphopeptide maps and radioiodinated peptide maps. These results indicate that native BBM vesicles contain a number of proteins that are phosphorylated by PKA when the PKA and ATP are present inside the vesicle space. One of these proteins appears to be identical to the 42-kDa protein that, as previously suggested by in vitro studies, acts as a regulatory cofactor mediating the inhibitory effect of PKA on the renal Na(+)-H+ exchanger.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1990

Volume

259

Issue

6 Pt 2

Start / End Page

F867 / F871

Location

United States

Related Subject Headings

  • Sodium-Hydrogen Exchangers
  • Rabbits
  • Protein Kinases
  • Phosphorylation
  • Phosphoproteins
  • Peptide Mapping
  • Microvilli
  • Magnesium
  • Kidney
  • Electrophoresis, Polyacrylamide Gel
 

Citation

APA
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MLA
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Morell, G., Steplock, D., Shenolikar, S., & Weinman, E. J. (1990). Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM. Am J Physiol, 259(6 Pt 2), F867–F871. https://doi.org/10.1152/ajprenal.1990.259.6.F867
Morell, G., D. Steplock, S. Shenolikar, and E. J. Weinman. “Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM.Am J Physiol 259, no. 6 Pt 2 (December 1990): F867–71. https://doi.org/10.1152/ajprenal.1990.259.6.F867.
Morell G, Steplock D, Shenolikar S, Weinman EJ. Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM. Am J Physiol. 1990 Dec;259(6 Pt 2):F867–71.
Morell, G., et al. “Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM.Am J Physiol, vol. 259, no. 6 Pt 2, Dec. 1990, pp. F867–71. Pubmed, doi:10.1152/ajprenal.1990.259.6.F867.
Morell G, Steplock D, Shenolikar S, Weinman EJ. Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM. Am J Physiol. 1990 Dec;259(6 Pt 2):F867–F871.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1990

Volume

259

Issue

6 Pt 2

Start / End Page

F867 / F871

Location

United States

Related Subject Headings

  • Sodium-Hydrogen Exchangers
  • Rabbits
  • Protein Kinases
  • Phosphorylation
  • Phosphoproteins
  • Peptide Mapping
  • Microvilli
  • Magnesium
  • Kidney
  • Electrophoresis, Polyacrylamide Gel