Skip to main content
Journal cover image

Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer

Publication ,  Journal Article
Castellano, RK; Craig, SL; Nuckolls, C; Rebek, J
Published in: Journal of the American Chemical Society
August 23, 2000

The kinetics and thermodynamics of multicomponent assembly in organic solution are investigated using fluorescence resonance energy transfer (FRET). Calix[4]arenes functionalized with ureas on their wider rims dimerize in organic solution, via intermolecular hydrogen bonding, to form encapsulation complexes. When one calixarene component is outfitted with a donor fluorophore and the other with an acceptor, dimerization brings the chromophores within 20 Å, a distance suitable for efficient energy transfer to occur between them. Excitation of the donor results in two colors of emitted light: one fluorescence band at the donor emission wavelength, and a second at the acceptor emission wavelength signaling the noncovalent union of three species - donor, acceptor, and small-molecule guest. By monitoring these wavelengths, assembly and dissociation processes are observed in real time at nanomolar concentrations. Rate and association constants for assembly processes are determined for the first time, and they reveal unexpected contributions from the structure and concentration of the monomer. Finally, the combination of FRET and the molecular recognition capabilities of the encapsulation complexes provides a sensitive and specific method for small-molecule sensing.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Journal of the American Chemical Society

DOI

ISSN

0002-7863

Publication Date

August 23, 2000

Volume

122

Issue

33

Start / End Page

7876 / 7882

Related Subject Headings

  • General Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Castellano, R. K., Craig, S. L., Nuckolls, C., & Rebek, J. (2000). Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer. Journal of the American Chemical Society, 122(33), 7876–7882. https://doi.org/10.1021/ja994397m
Castellano, R. K., S. L. Craig, C. Nuckolls, and J. Rebek. “Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer.” Journal of the American Chemical Society 122, no. 33 (August 23, 2000): 7876–82. https://doi.org/10.1021/ja994397m.
Castellano RK, Craig SL, Nuckolls C, Rebek J. Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer. Journal of the American Chemical Society. 2000 Aug 23;122(33):7876–82.
Castellano, R. K., et al. “Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer.” Journal of the American Chemical Society, vol. 122, no. 33, Aug. 2000, pp. 7876–82. Scopus, doi:10.1021/ja994397m.
Castellano RK, Craig SL, Nuckolls C, Rebek J. Detection and mechanistic studies of multicomponent assembly by fluorescence resonance energy transfer. Journal of the American Chemical Society. 2000 Aug 23;122(33):7876–7882.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

ISSN

0002-7863

Publication Date

August 23, 2000

Volume

122

Issue

33

Start / End Page

7876 / 7882

Related Subject Headings

  • General Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences