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The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis.

Publication ,  Journal Article
Yamaguchi, S; Sun, TP; Kawaide, H; Kamiya, Y
Published in: Plant physiology
April 1998

The ga2 mutant of Arabidopsis thaliana is a gibberellin-deficient dwarf. Previous biochemical studies have suggested that the ga2 mutant is impaired in the conversion of copalyl diphosphate to ent-kaurene, which is catalyzed by ent-kaurene synthase (KS). Overexpression of the previously isolated KS cDNA from pumpkin (Cucurbita maxima) (CmKS) in the ga2 mutant was able to complement the mutant phenotype. A genomic clone coding for KS, AtKS, was isolated from A. thaliana using CmKS cDNA as a heterologous probe. The corresponding A. thaliana cDNA was isolated and expressed in Escherichia coli as a fusion protein. The fusion protein showed enzymatic activity that converted [3H]copalyl diphosphate to [3H]ent-kaurene. The recombinant AtKS protein derived from the ga2-1 mutant is truncated by 14 kD at the C-terminal end and does not contain significant KS activity in vitro. Sequence analysis revealed that a C-2099 to T base substitution, which converts Gln-678 codon to a stop codon, is present in the AtKS cDNA from the ga2-1 mutant. Taken together, our results show that the GA2 locus encodes KS.

Duke Scholars

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

April 1998

Volume

116

Issue

4

Start / End Page

1271 / 1278

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Plant Biology & Botany
  • Molecular Sequence Data
  • Gibberellins
  • Genetic Complementation Test
  • Gene Expression Regulation, Plant
  • Gene Expression Regulation, Enzymologic
  • Escherichia coli
  • DNA, Complementary
  • Cloning, Molecular
 

Citation

APA
Chicago
ICMJE
MLA
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Yamaguchi, S., Sun, T. P., Kawaide, H., & Kamiya, Y. (1998). The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant Physiology, 116(4), 1271–1278. https://doi.org/10.1104/pp.116.4.1271
Yamaguchi, S., T. P. Sun, H. Kawaide, and Y. Kamiya. “The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis.Plant Physiology 116, no. 4 (April 1998): 1271–78. https://doi.org/10.1104/pp.116.4.1271.
Yamaguchi S, Sun TP, Kawaide H, Kamiya Y. The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant physiology. 1998 Apr;116(4):1271–8.
Yamaguchi, S., et al. “The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis.Plant Physiology, vol. 116, no. 4, Apr. 1998, pp. 1271–78. Epmc, doi:10.1104/pp.116.4.1271.
Yamaguchi S, Sun TP, Kawaide H, Kamiya Y. The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant physiology. 1998 Apr;116(4):1271–1278.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

April 1998

Volume

116

Issue

4

Start / End Page

1271 / 1278

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Plant Biology & Botany
  • Molecular Sequence Data
  • Gibberellins
  • Genetic Complementation Test
  • Gene Expression Regulation, Plant
  • Gene Expression Regulation, Enzymologic
  • Escherichia coli
  • DNA, Complementary
  • Cloning, Molecular