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The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway.

Publication ,  Journal Article
Silverstone, AL; Ciampaglio, CN; Sun, T
Published in: The Plant cell
February 1998

The recessive rga mutation is able to partially suppress phenotypic defects of the Arabidopsis gibberellin (GA) biosynthetic mutant ga1-3. Defects in stem elongation, flowering time, and leaf abaxial trichome initiation are suppressed by rga. This indicates that RGA is a negative regulator of the GA signal transduction pathway. We have identified 10 additional alleles of rga from a fast-neutron mutagenized ga1-3 population and used them to isolate the RGA gene by genomic subtraction. Our data suggest that RGA may be functioning as a transcriptional regulator. RGA was found to be a member of the VHIID regulatory family, which includes the radial root organizing gene SCARECROW and another GA signal transduction repressor, GAI. RGA and GAI proteins share a high degree of homology, but their N termini are more divergent. The presence of several structural features, including homopolymeric serine and threonine residues, a putative nuclear localization signal, leucine heptad repeats, and an LXXLL motif, indicates that the RGA protein may be a transcriptional regulator that represses the GA response. In support of the putative nuclear localization signal, we demonstrated that a transiently expressed green fluorescent protein-RGA fusion protein is localized to the nucleus in onion epidermal cells. Because the rga mutation abolished the high level of expression of the GA biosynthetic gene GA4 in the ga1-3 mutant background, we conclude that RGA may also play a role in controlling GA biosynthesis.

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Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

February 1998

Volume

10

Issue

2

Start / End Page

155 / 169

Related Subject Headings

  • Transcription, Genetic
  • Signal Transduction
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • Plant Proteins
  • Plant Biology & Botany
  • Mutagenesis
  • Molecular Sequence Data
  • Luminescent Proteins
 

Citation

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Silverstone, A. L., Ciampaglio, C. N., & Sun, T. (1998). The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. The Plant Cell, 10(2), 155–169. https://doi.org/10.1105/tpc.10.2.155
Silverstone, A. L., C. N. Ciampaglio, and T. Sun. “The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway.The Plant Cell 10, no. 2 (February 1998): 155–69. https://doi.org/10.1105/tpc.10.2.155.
Silverstone AL, Ciampaglio CN, Sun T. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. The Plant cell. 1998 Feb;10(2):155–69.
Silverstone, A. L., et al. “The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway.The Plant Cell, vol. 10, no. 2, Feb. 1998, pp. 155–69. Epmc, doi:10.1105/tpc.10.2.155.
Silverstone AL, Ciampaglio CN, Sun T. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. The Plant cell. 1998 Feb;10(2):155–169.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

February 1998

Volume

10

Issue

2

Start / End Page

155 / 169

Related Subject Headings

  • Transcription, Genetic
  • Signal Transduction
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • Plant Proteins
  • Plant Biology & Botany
  • Mutagenesis
  • Molecular Sequence Data
  • Luminescent Proteins