Skip to main content

TRPV1 receptors mediate particulate matter-induced apoptosis.

Publication ,  Journal Article
Agopyan, N; Head, J; Yu, S; Simon, SA
Published in: Am J Physiol Lung Cell Mol Physiol
March 2004

Exposure to airborne particulate matter (PM) is a world-wide health problem mainly because it produces adverse cardiovascular and respiratory effects that frequently result in morbidity. Despite many years of epidemiological and basic research, the mechanisms underlying PM toxicity remain largely unknown. To understand some of these mechanisms, we measured PM-induced apoptosis and necrosis in normal human airway epithelial cells and sensory neurons from both wild-type mice and mice lacking TRPV1 receptors using Alexa Fluor 488-conjugated annexin V and propidium iodide labeling, respectively. Exposure of environmental PMs containing residual oil fly ash and ash from Mount St. Helens was found to induce apoptosis, but not necrosis, as a consequence of sustained calcium influx through TRPV1 receptors. Apoptosis was completely prevented by inhibiting TRPV1 receptors with capsazepine or by removing extracellular calcium or in sensory neurons from TRPV1(-/-) mice. Binding of either one of the PMs to the cell membrane induced a capsazepine-sensitive increase in cAMP. PM-induced apoptosis was augmented upon the inhibition of PKA. PKA inhibition on its own also induced apoptosis, thereby suggesting that this pathway may be endogenously protective against apoptosis. In summary, it was found that inhibiting TRPV1 receptors prevents PM-induced apoptosis, thereby providing a potential mechanism to reduce their toxicity.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

March 2004

Volume

286

Issue

3

Start / End Page

L563 / L572

Location

United States

Related Subject Headings

  • Volcanic Eruptions
  • Respiratory System
  • Receptors, Drug
  • Neurons, Afferent
  • Necrosis
  • Mice, Knockout
  • Mice
  • Lung
  • Epithelial Cells
  • Cyclic AMP-Dependent Protein Kinases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Agopyan, N., Head, J., Yu, S., & Simon, S. A. (2004). TRPV1 receptors mediate particulate matter-induced apoptosis. Am J Physiol Lung Cell Mol Physiol, 286(3), L563–L572. https://doi.org/10.1152/ajplung.00299.2003
Agopyan, N., J. Head, S. Yu, and S. A. Simon. “TRPV1 receptors mediate particulate matter-induced apoptosis.Am J Physiol Lung Cell Mol Physiol 286, no. 3 (March 2004): L563–72. https://doi.org/10.1152/ajplung.00299.2003.
Agopyan N, Head J, Yu S, Simon SA. TRPV1 receptors mediate particulate matter-induced apoptosis. Am J Physiol Lung Cell Mol Physiol. 2004 Mar;286(3):L563–72.
Agopyan, N., et al. “TRPV1 receptors mediate particulate matter-induced apoptosis.Am J Physiol Lung Cell Mol Physiol, vol. 286, no. 3, Mar. 2004, pp. L563–72. Pubmed, doi:10.1152/ajplung.00299.2003.
Agopyan N, Head J, Yu S, Simon SA. TRPV1 receptors mediate particulate matter-induced apoptosis. Am J Physiol Lung Cell Mol Physiol. 2004 Mar;286(3):L563–L572.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

March 2004

Volume

286

Issue

3

Start / End Page

L563 / L572

Location

United States

Related Subject Headings

  • Volcanic Eruptions
  • Respiratory System
  • Receptors, Drug
  • Neurons, Afferent
  • Necrosis
  • Mice, Knockout
  • Mice
  • Lung
  • Epithelial Cells
  • Cyclic AMP-Dependent Protein Kinases