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Improved visualization of neurons labeled with horseradish peroxidase: silver-intensification of the pyrocatechol/p-phenylenediamine reaction product.

Publication ,  Journal Article
Fitzpatrick, D; Cheema, S; Merchenthaler, I; Rustioni, A
Published in: J Neurosci Methods
June 1983

A silver intensification procedure suitable for use with pyrocatechol/p-phenylenediamine (PC-PPD) product of the horseradish peroxidase (HRP) reaction is described. Qualitative and quantitative results from retrogradely labeled neurons in the cat cortex after thalamic injection of HRP demonstrate an increase of the intensity of labeling and in the number of darkly labeled cells after the intensification procedure. In both the non-intensified and the intensified PC-PPD reacted tissue the sensitivity was comparable to that of TMB-treated material. The ratio of lightly to darkly labeled neurons was very similar in intensified PC-PPD and TMB material, suggesting that the lightly labeled cells may have fewer terminals present at the level of the injected target.

Duke Scholars

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

June 1983

Volume

8

Issue

2

Start / End Page

161 / 169

Location

Netherlands

Related Subject Headings

  • Thalamic Nuclei
  • Phenylenediamines
  • Neurons
  • Neurology & Neurosurgery
  • Horseradish Peroxidase
  • Histological Techniques
  • Coloring Agents
  • Chromogenic Compounds
  • Cerebral Cortex
  • Cats
 

Citation

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ICMJE
MLA
NLM
Fitzpatrick, D., Cheema, S., Merchenthaler, I., & Rustioni, A. (1983). Improved visualization of neurons labeled with horseradish peroxidase: silver-intensification of the pyrocatechol/p-phenylenediamine reaction product. J Neurosci Methods, 8(2), 161–169. https://doi.org/10.1016/0165-0270(83)90117-6
Fitzpatrick, D., S. Cheema, I. Merchenthaler, and A. Rustioni. “Improved visualization of neurons labeled with horseradish peroxidase: silver-intensification of the pyrocatechol/p-phenylenediamine reaction product.J Neurosci Methods 8, no. 2 (June 1983): 161–69. https://doi.org/10.1016/0165-0270(83)90117-6.
Fitzpatrick, D., et al. “Improved visualization of neurons labeled with horseradish peroxidase: silver-intensification of the pyrocatechol/p-phenylenediamine reaction product.J Neurosci Methods, vol. 8, no. 2, June 1983, pp. 161–69. Pubmed, doi:10.1016/0165-0270(83)90117-6.
Journal cover image

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

June 1983

Volume

8

Issue

2

Start / End Page

161 / 169

Location

Netherlands

Related Subject Headings

  • Thalamic Nuclei
  • Phenylenediamines
  • Neurons
  • Neurology & Neurosurgery
  • Horseradish Peroxidase
  • Histological Techniques
  • Coloring Agents
  • Chromogenic Compounds
  • Cerebral Cortex
  • Cats