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Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II.

Publication ,  Journal Article
Greenleaf, AL; Borsett, LM; Jiamachello, PF; Coulter, DE
Published in: Cell
November 1979

Following EMS mutagenesis we recovered a mutant of D. melanogaster that grows at concentrations of alpha-amanitin lethal to wild-type. To our knowledge this mutant represents the first example of an amanitin-resistant eucaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA-dependent RNA polymerase II, which is approximately 250 times less sensitive to inhibition by amanitin than the wild-type polymerase II whether tested in nuclei, in partially-fractionated extracts or as a highly purified enzyme. While the wild-type enzyme activity is inhibited 50% by 2.1 x 10(-8) M alpha-amanitin, inhibition of 50% of the AmaC4 RNA polymerase II activity requires a toxin concentration of 5.6 x 10(-6) M. The mutation responsible for the amanitin resistance of AmaC4 is on the X chromosome near the vermillion locus.

Duke Scholars

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

November 1979

Volume

18

Issue

3

Start / End Page

613 / 622

Location

United States

Related Subject Headings

  • X Chromosome
  • RNA Polymerase II
  • Mutation
  • Male
  • Larva
  • Female
  • Drug Resistance
  • Drosophila melanogaster
  • Developmental Biology
  • DNA-Directed RNA Polymerases
 

Citation

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Greenleaf, A. L., Borsett, L. M., Jiamachello, P. F., & Coulter, D. E. (1979). Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II. Cell, 18(3), 613–622. https://doi.org/10.1016/0092-8674(79)90116-8
Greenleaf, A. L., L. M. Borsett, P. F. Jiamachello, and D. E. Coulter. “Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II.Cell 18, no. 3 (November 1979): 613–22. https://doi.org/10.1016/0092-8674(79)90116-8.
Greenleaf AL, Borsett LM, Jiamachello PF, Coulter DE. Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II. Cell. 1979 Nov;18(3):613–22.
Greenleaf, A. L., et al. “Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II.Cell, vol. 18, no. 3, Nov. 1979, pp. 613–22. Pubmed, doi:10.1016/0092-8674(79)90116-8.
Greenleaf AL, Borsett LM, Jiamachello PF, Coulter DE. Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II. Cell. 1979 Nov;18(3):613–622.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

November 1979

Volume

18

Issue

3

Start / End Page

613 / 622

Location

United States

Related Subject Headings

  • X Chromosome
  • RNA Polymerase II
  • Mutation
  • Male
  • Larva
  • Female
  • Drug Resistance
  • Drosophila melanogaster
  • Developmental Biology
  • DNA-Directed RNA Polymerases