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Dye-ligand affinity chromatography of RNA polymerase II.

Publication ,  Journal Article
Skripal, IG; Weeks, JR; Greenleaf, AL
Published in: Acta Biochim Biophys Hung
1986

The binding of wheat germ RNA polymerase II to five different dye-ligand chromatography gels (Matrex gels, Amicon Corp.) was tested. A quantitative binding of the enzyme to four of the gels, namely Dyematrex gels Blue A, Blue B, Red A and Green A was observed. Only the Orange A gel column failed to bind the enzyme strongly. Nearly 100% of the activity could be recovered from the Green A column by elution with high salt concentration and high pH. Under these conditions only a part of the activity was eluted from the other three columns since the enzyme bound tightly. Enzyme activity could be removed from the columns by elution with nucleotide substrates, but the yield from the Blue A, Blue B and Red A columns was still low (7 to 42%). The Green A Matrex gel appeared to be useful for the purification and analysis of RNA polymerase.

Duke Scholars

Published In

Acta Biochim Biophys Hung

ISSN

0237-6261

Publication Date

1986

Volume

21

Issue

3

Start / End Page

215 / 224

Location

Hungary

Related Subject Headings

  • Triticum
  • RNA Polymerase II
  • Electrophoresis, Polyacrylamide Gel
  • Coloring Agents
  • Chromatography, Agarose
  • Chromatography, Affinity
 

Citation

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MLA
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Skripal, I. G., Weeks, J. R., & Greenleaf, A. L. (1986). Dye-ligand affinity chromatography of RNA polymerase II. Acta Biochim Biophys Hung, 21(3), 215–224.
Skripal, I. G., J. R. Weeks, and A. L. Greenleaf. “Dye-ligand affinity chromatography of RNA polymerase II.Acta Biochim Biophys Hung 21, no. 3 (1986): 215–24.
Skripal IG, Weeks JR, Greenleaf AL. Dye-ligand affinity chromatography of RNA polymerase II. Acta Biochim Biophys Hung. 1986;21(3):215–24.
Skripal, I. G., et al. “Dye-ligand affinity chromatography of RNA polymerase II.Acta Biochim Biophys Hung, vol. 21, no. 3, 1986, pp. 215–24.
Skripal IG, Weeks JR, Greenleaf AL. Dye-ligand affinity chromatography of RNA polymerase II. Acta Biochim Biophys Hung. 1986;21(3):215–224.

Published In

Acta Biochim Biophys Hung

ISSN

0237-6261

Publication Date

1986

Volume

21

Issue

3

Start / End Page

215 / 224

Location

Hungary

Related Subject Headings

  • Triticum
  • RNA Polymerase II
  • Electrophoresis, Polyacrylamide Gel
  • Coloring Agents
  • Chromatography, Agarose
  • Chromatography, Affinity