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Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

Publication ,  Journal Article
Ingles, CJ; Biggs, J; Wong, JK; Weeks, JR; Greenleaf, AL
Published in: Proc Natl Acad Sci U S A
June 1983
Published version (DOI) Link to item

Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.

Duke Scholars

Arno Lee Greenleaf
Author Arno Lee Greenleaf Biochemistry

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.80.11.3396

ISSN

0027-8424

Publication Date

June 1983

Volume

80

Issue

11

Start / End Page

3396 / 3400

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Species Specificity
  • RNA Polymerase II
  • Peptides
  • Nucleic Acid Hybridization
  • Mutation
  • Mesocricetus
  • Genes
  • Drosophila melanogaster
  • DNA-Directed RNA Polymerases
 

Citation

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Ingles, C. J., Biggs, J., Wong, J. K., Weeks, J. R., & Greenleaf, A. L. (1983). Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species. Proc Natl Acad Sci U S A, 80(11), 3396–3400. https://doi.org/10.1073/pnas.80.11.3396
Ingles, C. J., J. Biggs, J. K. Wong, J. R. Weeks, and A. L. Greenleaf. “Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.” Proc Natl Acad Sci U S A 80, no. 11 (June 1983): 3396–3400. https://doi.org/10.1073/pnas.80.11.3396.
Ingles CJ, Biggs J, Wong JK, Weeks JR, Greenleaf AL. Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3396–400.
Ingles, C. J., et al. “Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.” Proc Natl Acad Sci U S A, vol. 80, no. 11, June 1983, pp. 3396–400. Pubmed, doi:10.1073/pnas.80.11.3396.
Ingles CJ, Biggs J, Wong JK, Weeks JR, Greenleaf AL. Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3396–3400.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.80.11.3396

ISSN

0027-8424

Publication Date

June 1983

Volume

80

Issue

11

Start / End Page

3396 / 3400

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Species Specificity
  • RNA Polymerase II
  • Peptides
  • Nucleic Acid Hybridization
  • Mutation
  • Mesocricetus
  • Genes
  • Drosophila melanogaster
  • DNA-Directed RNA Polymerases