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A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis.

Publication ,  Journal Article
Angerer, LM; Oleksyn, DW; Logan, CY; McClay, DR; Dale, L; Angerer, RC
Published in: Development (Cambridge, England)
March 2000

To examine whether a BMP signaling pathway functions in specification of cell fates in sea urchin embryos, we have cloned sea urchin BMP2/4, analyzed its expression in time and space in developing embryos and assayed the developmental consequences of changing its concentration through mRNA injection experiments. These studies show that BMP4 mRNAs accumulate transiently during blastula stages, beginning around the 200-cell stage, 14 hours postfertilization. Soon after the hatching blastula stage, BMP2/4 transcripts can be detected in presumptive ectoderm, where they are enriched on the oral side. Injection of BMP2/4 mRNA at the one-cell stage causes a dose-dependent suppression of commitment of cells to vegetal fates and ectoderm differentiates almost exclusively as a squamous epithelial tissue. In contrast, NOGGIN, an antagonist of BMP2/4, enhances differentiation of endoderm, a vegetal tissue, and promotes differentiation of cells characteristic of the ciliated band, which contains neurogenic ectoderm. These findings support a model in which the balance of BMP2/4 signals produced by animal cell progeny and opposing vegetalizing signals sent during cleavage stages regulate the position of the ectoderm/ endoderm boundary. In addition, BMP2/4 levels influence the decision within ectoderm between epidermal and nonepidermal differentiation.

Duke Scholars

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2000

Volume

127

Issue

5

Start / End Page

1105 / 1114

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Transforming Growth Factor beta
  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Sea Urchins
  • Recombinant Proteins
  • RNA, Messenger
  • Oocytes
 

Citation

APA
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MLA
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Angerer, L. M., Oleksyn, D. W., Logan, C. Y., McClay, D. R., Dale, L., & Angerer, R. C. (2000). A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. Development (Cambridge, England), 127(5), 1105–1114. https://doi.org/10.1242/dev.127.5.1105
Angerer, L. M., D. W. Oleksyn, C. Y. Logan, D. R. McClay, L. Dale, and R. C. Angerer. “A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis.Development (Cambridge, England) 127, no. 5 (March 2000): 1105–14. https://doi.org/10.1242/dev.127.5.1105.
Angerer LM, Oleksyn DW, Logan CY, McClay DR, Dale L, Angerer RC. A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. Development (Cambridge, England). 2000 Mar;127(5):1105–14.
Angerer, L. M., et al. “A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis.Development (Cambridge, England), vol. 127, no. 5, Mar. 2000, pp. 1105–14. Epmc, doi:10.1242/dev.127.5.1105.
Angerer LM, Oleksyn DW, Logan CY, McClay DR, Dale L, Angerer RC. A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. Development (Cambridge, England). 2000 Mar;127(5):1105–1114.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2000

Volume

127

Issue

5

Start / End Page

1105 / 1114

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Transforming Growth Factor beta
  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Sea Urchins
  • Recombinant Proteins
  • RNA, Messenger
  • Oocytes