Scholars@Duke

Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice.

Publication ,  Journal Article
Zhao, J; Shi, W; Wang, Y-L; Chen, H; Bringas, P; Datto, MB; Frederick, JP; Wang, X-F; Warburton, D
Published in: Am J Physiol Lung Cell Mol Physiol
March 2002
Published version (DOI) Link to item

Transforming growth factor-beta (TGF-beta) signaling plays an important regulatory role during lung fibrogenesis. Smad3 was identified in the pathway for transducing TGF-beta signals from the cell membrane to the nucleus. Using mice without Smad3 gene expression, we investigated whether Smad3 could regulate bleomycin-induced pulmonary fibrosis in vivo. Mice deficient in Smad3 demonstrated suppressed type I procollagen mRNA expression and reduced hydroxyproline content in the lungs compared with wild-type mice treated with bleomycin. Furthermore, loss of Smad3 greatly attenuated morphological fibrotic responses to bleomycin in the mouse lungs, suggesting that Smad3 is implicated in the pathogenesis of pulmonary fibrosis. These results show that Smad3 contributes to bleomycin-induced lung injury and that Smad3 may serve as a novel target for potential therapeutic treatment of lung fibrosis.

Duke Scholars

Michael Bradley Datto
Author Michael Bradley Datto Pathology
Xiao-Fan Wang
Author Xiao-Fan Wang Pharmacology & Cancer Biology
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Published In

Am J Physiol Lung Cell Mol Physiol

DOI

10.1152/ajplung.00151.2001

ISSN

1040-0605

Publication Date

March 2002

Volume

282

Issue

3

Start / End Page

L585 / L593

Location

United States

Related Subject Headings

  • Trans-Activators
  • Smad3 Protein
  • Respiratory System
  • Pulmonary Fibrosis
  • Mice, Knockout
  • Mice
  • Lung
  • Fibronectins
  • DNA-Binding Proteins
  • Collagen
 

Citation

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Zhao, J., Shi, W., Wang, Y.-L., Chen, H., Bringas, P., Datto, M. B., … Warburton, D. (2002). Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Physiol Lung Cell Mol Physiol, 282(3), L585–L593. https://doi.org/10.1152/ajplung.00151.2001
Zhao, Jingsong, Wei Shi, Yan-Ling Wang, Hui Chen, Pablo Bringas, Michael B. Datto, Joshua P. Frederick, Xiao-Fan Wang, and David Warburton. “Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice.” Am J Physiol Lung Cell Mol Physiol 282, no. 3 (March 2002): L585–93. https://doi.org/10.1152/ajplung.00151.2001.
Zhao J, Shi W, Wang Y-L, Chen H, Bringas P, Datto MB, et al. Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L585–93.
Zhao, Jingsong, et al. “Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice.” Am J Physiol Lung Cell Mol Physiol, vol. 282, no. 3, Mar. 2002, pp. L585–93. Pubmed, doi:10.1152/ajplung.00151.2001.
Zhao J, Shi W, Wang Y-L, Chen H, Bringas P, Datto MB, Frederick JP, Wang X-F, Warburton D. Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L585–L593.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

10.1152/ajplung.00151.2001

ISSN

1040-0605

Publication Date

March 2002

Volume

282

Issue

3

Start / End Page

L585 / L593

Location

United States

Related Subject Headings

  • Trans-Activators
  • Smad3 Protein
  • Respiratory System
  • Pulmonary Fibrosis
  • Mice, Knockout
  • Mice
  • Lung
  • Fibronectins
  • DNA-Binding Proteins
  • Collagen