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TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract.

Publication ,  Journal Article
Yang, HK; Linnoila, RI; Conrad, NK; Krasna, MJ; Aisner, SC; Johnson, BE; Kelley, MJ
Published in: Int J Cancer
August 22, 1995

Patients who initially develop an upper aerodigestive tract cancer have an increased risk of second primary cancers. We examined TP53 and RAS mutations and p53 protein in 21 tumors from 10 patients with upper aerodigestive tract cancer who developed a metachronous tumor, to assess the genetic changes that occur in multiple primary tumors from the same individual. Thirteen of 21 (62%) tumors were found to have mis-sense mutations of either TP53 or RAS. Six tumors had TP53 mutations in codons 5 to 8 and 10 tumors from 7 patients had mutations of codons 12 or 13 of K-RAS. Only one patient had concordance of a mutation in 2 tumors; this mutation occurred in K-RAS and was accompanied by discordance of TP53 mutation. Three patients had tumors discordant for both TP53 and RAS mutations. Smoking-related tumors had TP53 and RAS mutations which were transversions in 11 (9 G:C to T:A and 2 G:C to C:G) and transitions in 3 (2 G:C to A:T and 1 A:T to G:C). Tumors not associated with smoking contained only transitions (both G:C to A:T). p53 protein was detected by immunohistochemistry in 7 of 13 (54%) tumors and was concordant in the multiple tumors of 3 patients. Three of the 7 tumors staining for p53 also had TP53 mutations. Thus, genetic alterations are discordant in multiple primary cancers and the pattern of mutations is similar to that found in patients with a single primary tumor, supporting the concept that these cancers arise independently.

Duke Scholars

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

August 22, 1995

Volume

64

Issue

4

Start / End Page

229 / 233

Location

United States

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Stomach Neoplasms
  • Polymorphism, Restriction Fragment Length
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Middle Aged
  • Male
  • Lung Neoplasms
  • Laryngeal Neoplasms
 

Citation

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Yang, H. K., Linnoila, R. I., Conrad, N. K., Krasna, M. J., Aisner, S. C., Johnson, B. E., & Kelley, M. J. (1995). TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract. Int J Cancer, 64(4), 229–233. https://doi.org/10.1002/ijc.2910640403
Yang, H. K., R. I. Linnoila, N. K. Conrad, M. J. Krasna, S. C. Aisner, B. E. Johnson, and M. J. Kelley. “TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract.Int J Cancer 64, no. 4 (August 22, 1995): 229–33. https://doi.org/10.1002/ijc.2910640403.
Yang HK, Linnoila RI, Conrad NK, Krasna MJ, Aisner SC, Johnson BE, et al. TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract. Int J Cancer. 1995 Aug 22;64(4):229–33.
Yang, H. K., et al. “TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract.Int J Cancer, vol. 64, no. 4, Aug. 1995, pp. 229–33. Pubmed, doi:10.1002/ijc.2910640403.
Yang HK, Linnoila RI, Conrad NK, Krasna MJ, Aisner SC, Johnson BE, Kelley MJ. TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract. Int J Cancer. 1995 Aug 22;64(4):229–233.
Journal cover image

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

August 22, 1995

Volume

64

Issue

4

Start / End Page

229 / 233

Location

United States

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Stomach Neoplasms
  • Polymorphism, Restriction Fragment Length
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Middle Aged
  • Male
  • Lung Neoplasms
  • Laryngeal Neoplasms