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Magnetic resonance microscopy of mouse embryos.

Publication ,  Journal Article
Smith, BR; Johnson, GA; Groman, EV; Linney, E
Published in: Proc Natl Acad Sci U S A
April 26, 1994

The increased use of the mouse as a model for various aspects of mammalian biology has caused a renewed interest in developing strategies for examining and comparing normal and abnormal mouse embryonic development and anatomy. In this study, we have explored the use of magnetic resonance microscopy as a tool for these purposes. Techniques for the fixation, embedding, perfusion, and image acquisition of mouse embryos are described. The perfusion of bovine serum albumin-diethylenetriamine pentaacetic anhydride-gadolinium as a contrast agent enhances images of the developing embryonic vasculature during critical stages of organogenesis and allows for comparisons when embryos have been treated with teratogens such as retinoic acid. The acquired three-dimensional data sets are available for archiving, distributing, and postacquisition manipulations such as computer segmentation of anatomical structures.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 26, 1994

Volume

91

Issue

9

Start / End Page

3530 / 3533

Location

United States

Related Subject Headings

  • Pentetic Acid
  • Organometallic Compounds
  • Mice
  • Image Processing, Computer-Assisted
  • Gadolinium DTPA
  • Contrast Media
  • Animals
  • Albumins
  • Age Factors
 

Citation

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Smith, B. R., Johnson, G. A., Groman, E. V., & Linney, E. (1994). Magnetic resonance microscopy of mouse embryos. Proc Natl Acad Sci U S A, 91(9), 3530–3533. https://doi.org/10.1073/pnas.91.9.3530
Smith, B. R., G. A. Johnson, E. V. Groman, and E. Linney. “Magnetic resonance microscopy of mouse embryos.Proc Natl Acad Sci U S A 91, no. 9 (April 26, 1994): 3530–33. https://doi.org/10.1073/pnas.91.9.3530.
Smith BR, Johnson GA, Groman EV, Linney E. Magnetic resonance microscopy of mouse embryos. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3530–3.
Smith, B. R., et al. “Magnetic resonance microscopy of mouse embryos.Proc Natl Acad Sci U S A, vol. 91, no. 9, Apr. 1994, pp. 3530–33. Pubmed, doi:10.1073/pnas.91.9.3530.
Smith BR, Johnson GA, Groman EV, Linney E. Magnetic resonance microscopy of mouse embryos. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3530–3533.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 26, 1994

Volume

91

Issue

9

Start / End Page

3530 / 3533

Location

United States

Related Subject Headings

  • Pentetic Acid
  • Organometallic Compounds
  • Mice
  • Image Processing, Computer-Assisted
  • Gadolinium DTPA
  • Contrast Media
  • Animals
  • Albumins
  • Age Factors