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Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells.

Publication ,  Journal Article
Bahnson, TD; Pandol, SJ; Dionne, VE
Published in: J Biol Chem
May 25, 1993

In the pancreatic acinar cell, hormonal stimulation causes a rise in the intracellular free Ca2+ concentration by activating the inositol 1,4,5-trisphosphate-mediated release of Ca2+ from intracellular stores (Berridge, M. J., and Irvine, R. F. (1989) Nature 341, 197-205). The released Ca2+ is, for the most part, extruded from the cell, necessitating a mechanism for Ca2+ entry and reloading of intracellular Ca2+ stores (Putney, J. W., Jr. (1990) Cell Calcium 11, 611-624; Rink, T. J. (1990) FEBS Lett. 268, 381-385). However, neither the mechanism of depletion-activated Ca2+ entry nor the signal that activates it is known. We report here that a sustained inward current of depletion-activated Ca2+ entry can be measured in pancreatic acinar cells using patch-clamp recording methods. Furthermore, the current can be blocked by an inhibitor of guanylyl cyclase, can be reactivated by 8-bromo-cGMP after inhibition, and can be activated in the absence of Ca2+ depletion by perfusing the cell with cGMP, but not cAMP. Intracellular perfusion with 1,3,4,5-inositol tetrakisphosphate did not activate an inward current, whereas perfusion with 2,4,5-inositol trisphosphate did activate an inward current. We conclude that cGMP may be an intracellular messenger that regulates depletion-activated Ca2+ entry.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 25, 1993

Volume

268

Issue

15

Start / End Page

10808 / 10812

Location

United States

Related Subject Headings

  • Time Factors
  • Second Messenger Systems
  • Rats, Sprague-Dawley
  • Rats
  • Pancreas
  • Models, Biological
  • Membrane Potentials
  • Kinetics
  • Inositol Phosphates
  • Inositol 1,4,5-Trisphosphate
 

Citation

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Bahnson, T. D., Pandol, S. J., & Dionne, V. E. (1993). Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. J Biol Chem, 268(15), 10808–10812.
Bahnson, T. D., S. J. Pandol, and V. E. Dionne. “Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells.J Biol Chem 268, no. 15 (May 25, 1993): 10808–12.
Bahnson TD, Pandol SJ, Dionne VE. Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. J Biol Chem. 1993 May 25;268(15):10808–12.
Bahnson, T. D., et al. “Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells.J Biol Chem, vol. 268, no. 15, May 1993, pp. 10808–12.
Bahnson TD, Pandol SJ, Dionne VE. Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. J Biol Chem. 1993 May 25;268(15):10808–10812.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 25, 1993

Volume

268

Issue

15

Start / End Page

10808 / 10812

Location

United States

Related Subject Headings

  • Time Factors
  • Second Messenger Systems
  • Rats, Sprague-Dawley
  • Rats
  • Pancreas
  • Models, Biological
  • Membrane Potentials
  • Kinetics
  • Inositol Phosphates
  • Inositol 1,4,5-Trisphosphate