Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase.
Publication
, Journal Article
Turner, MA; Achyuthan, AM; Hershfield, MS; McInnes, RR; Howell, PL
Published in: J Mol Biol
June 3, 1994
Human argininosuccinic acid lyase (ASAL) has been expressed, purified and crystallized in several distinct crystal morphologies. At present only one form is suitable for X-ray diffraction analysis. These crystals grow as hexagonal prisms, with unit cell dimensions a = b = 104.6 A, c = 185.3 A and alpha = beta = 90 degrees, gamma = 120 degrees. The crystals exhibit the symmetry of space group P3(1)21 or its enantiomorph, P3(2)21 (indistinguishable crystallographically) and diffract to a minimum d-spacing of approximately 3.5 A.
Duke Scholars
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
June 3, 1994
Volume
239
Issue
2
Start / End Page
336 / 338
Location
Netherlands
Related Subject Headings
- Recombinant Proteins
- Protein Conformation
- Multigene Family
- Humans
- Crystallography, X-Ray
- Crystallization
- Biochemistry & Molecular Biology
- Argininosuccinate Lyase
- 3107 Microbiology
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Turner, M. A., Achyuthan, A. M., Hershfield, M. S., McInnes, R. R., & Howell, P. L. (1994). Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase. J Mol Biol, 239(2), 336–338. https://doi.org/10.1006/jmbi.1994.1372
Turner, M. A., A. M. Achyuthan, M. S. Hershfield, R. R. McInnes, and P. L. Howell. “Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase.” J Mol Biol 239, no. 2 (June 3, 1994): 336–38. https://doi.org/10.1006/jmbi.1994.1372.
Turner MA, Achyuthan AM, Hershfield MS, McInnes RR, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase. J Mol Biol. 1994 Jun 3;239(2):336–8.
Turner, M. A., et al. “Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase.” J Mol Biol, vol. 239, no. 2, June 1994, pp. 336–38. Pubmed, doi:10.1006/jmbi.1994.1372.
Turner MA, Achyuthan AM, Hershfield MS, McInnes RR, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase. J Mol Biol. 1994 Jun 3;239(2):336–338.
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
June 3, 1994
Volume
239
Issue
2
Start / End Page
336 / 338
Location
Netherlands
Related Subject Headings
- Recombinant Proteins
- Protein Conformation
- Multigene Family
- Humans
- Crystallography, X-Ray
- Crystallization
- Biochemistry & Molecular Biology
- Argininosuccinate Lyase
- 3107 Microbiology
- 3101 Biochemistry and cell biology