Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis.
Apoptotic DNA fragmentation and chromatin condensation are mediated by the caspase-activated DFF40/ CAD nuclease, which is chaperoned and inhibited by DFF45/ICAD. CIDE proteins share a homologous regulatory CIDE-N domain with DFF40/CAD and DFF45/ ICAD. Here we report the solution structure of CIDE-N of human CIDE-B. We show that the CIDE-N of CIDE-B interacts with CIDE-N domains of both DFF40 and DFF45. The binding epitopes are similar and map to a highly charged bipolar surface region of CIDE-B. Furthermore, we demonstrate that the CIDE-N of CIDE-B regulates enzymatic activity of the DFF40/ DFF45 complex in vitro. Based on these results and mutagenesis data, we propose a model for the CIDE-N/ CIDE-N complex and discuss the role of this novel bipolar interaction in mediating downstream events of apoptosis.
Duke Scholars
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Related Subject Headings
- Sequence Alignment
- Proteins
- Protein Structure, Tertiary
- Protein Structure, Secondary
- Protein Binding
- Molecular Sequence Data
- Models, Molecular
- Developmental Biology
- Deoxyribonucleases
- DNA Fragmentation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Sequence Alignment
- Proteins
- Protein Structure, Tertiary
- Protein Structure, Secondary
- Protein Binding
- Molecular Sequence Data
- Models, Molecular
- Developmental Biology
- Deoxyribonucleases
- DNA Fragmentation