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Hormonal and metabolic regulation of human chondrosarcoma in vitro.

Publication ,  Journal Article
McCumbee, WD; Harrelson, JM; Lebovitz, HE
Published in: Cancer Res
February 1983

Prostaglandin A1 has a profound inhibitory effect on uridine incorporation into RNA of normal cartilage whereas N6-monobutyryladenosine 3',5'-cyclic monophosphate is either stimulatory or without an effect. Sera from intact and growth hormone-treated hypophysectomized rats stimulate RNA synthesis but serum from untreated hypophysectomized rats does not. The present study investigated the in vitro regulation of [3H]uridine incorporation into RNA of six human chondrosarcomas to determine if malignant human chondrocytes are under similar metabolic and hormonal regulation. Prostaglandin A1 (25 micrograms/ml) markedly inhibited uridine incorporation in all six tumors (56 to 80%). N6-Monobutyryladenosine 3',5'-cyclic monophosphate (1 mM) inhibited uridine incorporation in five tumors (20 to 50%). Uridine incorporation was stimulated by growth hormone-dependent serum factors in one tumor and by growth hormone-independent serum factors in two tumors. Two tumors were more responsive to serum from growth hormone-treated hypophysectomized rats than to serum from intact rats, and one tumor was unresponsive to serum stimulation. The data indicate that: (a) prostaglandin A1 is a very potent inhibitor of RNA synthesis in human chondrosarcomas; (b) N6-monobutyryladenosine 3',5'-cyclic monophosphate affects human chondrosarcomas differently than it does normal cartilage; and (c) responses of human chondrosarcomas to serum growth factors vary among individual tumors.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1983

Volume

43

Issue

2

Start / End Page

513 / 516

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Prostaglandins A
  • Organ Culture Techniques
  • Oncology & Carcinogenesis
  • Humans
  • Growth Hormone
  • Culture Media
  • Chondrosarcoma
  • Cell Survival
  • Cell Division
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McCumbee, W. D., Harrelson, J. M., & Lebovitz, H. E. (1983). Hormonal and metabolic regulation of human chondrosarcoma in vitro. Cancer Res, 43(2), 513–516.
McCumbee, W. D., J. M. Harrelson, and H. E. Lebovitz. “Hormonal and metabolic regulation of human chondrosarcoma in vitro.Cancer Res 43, no. 2 (February 1983): 513–16.
McCumbee WD, Harrelson JM, Lebovitz HE. Hormonal and metabolic regulation of human chondrosarcoma in vitro. Cancer Res. 1983 Feb;43(2):513–6.
McCumbee, W. D., et al. “Hormonal and metabolic regulation of human chondrosarcoma in vitro.Cancer Res, vol. 43, no. 2, Feb. 1983, pp. 513–16.
McCumbee WD, Harrelson JM, Lebovitz HE. Hormonal and metabolic regulation of human chondrosarcoma in vitro. Cancer Res. 1983 Feb;43(2):513–516.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1983

Volume

43

Issue

2

Start / End Page

513 / 516

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Prostaglandins A
  • Organ Culture Techniques
  • Oncology & Carcinogenesis
  • Humans
  • Growth Hormone
  • Culture Media
  • Chondrosarcoma
  • Cell Survival
  • Cell Division