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Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus.

Publication ,  Journal Article
Mills, E; Kuhn, CM; Feinglos, MN; Surwit, R
Published in: Am J Physiol
January 1993

The C57BL/6J (BL/6) mouse develops non-insulin-dependent diabetes mellitus (NIDDM) when fed a high fat-high simple carbohydrate (HFHSC) diet, whereas A/J mice do not. The purpose of the study was to determine whether hypertension occurred with NIDDM and whether it was sustained by sympathetic nervous system (SNS) hyperactivity. After 3 mo on an HFHSC diet with a low Na content (0.06%), awake, tail-cuff systolic blood pressure (BP) increased 20% above the control diet in BL/6 (138 +/- 3 vs. 115 +/- 4) but not in A/J (115 +/- 6 vs. 113 +/- 2 mmHg) mice. On a normal Na (0.4%)-HFHSC diet, BL/6 mice had a higher BP than on 0.06% Na (149 +/- 3 at 3 mo, 162 +/- 6 at 4.5 mo). After 1 mo on the 0.06% Na-HFHSC diet, direct BP of anesthetized BL/6 mice was 18% higher than control. The hypotensive response to interruption of SNS activity by ganglionic blockade (chlorisondamine) increased in the BL/6 mice (50%), whereas the heart rate response increased in both strains (20-30%). Analysis of variance (ANOVA) on glucose detected significant effects of strain and diet and a strain x diet interaction (P = 0.0007). At 1 or 3 mo, HFHSC-fed BL/6 mice were hyperglycemic (> 11 mM) compared with diet or strain controls. The ANOVA on insulin detected strain and diet effects but not a strain x diet interaction (P = 0.3). HFHSC increased insulin above the control of 140-160 pM in A/J and BL/6 strain (20-70% at 1 mo, 400% at 3 mo).(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1993

Volume

264

Issue

1 Pt 2

Start / End Page

R73 / R78

Location

United States

Related Subject Headings

  • Wakefulness
  • Urethane
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Insulin
  • Hypertension
  • Heart Rate
  • Diabetic Angiopathies
 

Citation

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MLA
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Mills, E., Kuhn, C. M., Feinglos, M. N., & Surwit, R. (1993). Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus. Am J Physiol, 264(1 Pt 2), R73–R78. https://doi.org/10.1152/ajpregu.1993.264.1.R73
Mills, E., C. M. Kuhn, M. N. Feinglos, and R. Surwit. “Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus.Am J Physiol 264, no. 1 Pt 2 (January 1993): R73–78. https://doi.org/10.1152/ajpregu.1993.264.1.R73.
Mills E, Kuhn CM, Feinglos MN, Surwit R. Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus. Am J Physiol. 1993 Jan;264(1 Pt 2):R73–8.
Mills, E., et al. “Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus.Am J Physiol, vol. 264, no. 1 Pt 2, Jan. 1993, pp. R73–78. Pubmed, doi:10.1152/ajpregu.1993.264.1.R73.
Mills E, Kuhn CM, Feinglos MN, Surwit R. Hypertension in CB57BL/6J mouse model of non-insulin-dependent diabetes mellitus. Am J Physiol. 1993 Jan;264(1 Pt 2):R73–R78.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1993

Volume

264

Issue

1 Pt 2

Start / End Page

R73 / R78

Location

United States

Related Subject Headings

  • Wakefulness
  • Urethane
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Insulin
  • Hypertension
  • Heart Rate
  • Diabetic Angiopathies