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Evaluation of selective liver denervation methods.

Publication ,  Journal Article
Cucchiaro, G; Yamaguchi, Y; Mills, E; Kuhn, CM; Anthony, DC; Branum, GD; Epstein, R; Meyers, WC
Published in: Am J Physiol
November 1990

This study compares four methods of hepatic denervation and defines the rate and physiological significance of reinnervation. Five groups of rats were prepared: 10 underwent orthotopic liver transplantation. In nine rats a 90% aqueous phenol solution was applied circumferentially to the portal vein. Thirteen rats underwent microsurgical denervation; 28 received different doses of 6-hydroxydopamine (6-HODA) administered as a single intraportal injection [50 (n = 10), 75 (n = 6), and 100 mg/kg (n = 6)]. Twelve rats were studied as controls. Rats were killed 1, 4, and 8 wk after surgery to determine liver tissue content of norepinephrine (NE). Changes in mean arterial pressure (MAP) in response to hepatic nerve stimulation, which was supramaximum in intensity and frequency, were measured before rats were killed. NE content in controls ranged from 121 to 204 ng/g and MAP increased by 30-38 mmHg after electrical stimulation. At 1, 4, and 8 wk after treatment the liver NE content was less than 1, 2.3, and 20.2 ng/g in the transplant group; less than 1, 2.7, 4.1 ng/g in the phenol group; and 17.2, less than 1, and 3 ng/g in the surgically denervated group. In the 6-HODA group, values were 18.9, 47, and 61.5 ng/g (50 mg/kg); 5.7, 20.2, and 15 ng/g (75 mg/kg); and 7.7, 2.5, and 17.5 ng/g (100 mg/kg). When the level of NE was undetectable, MAP increase after stimulation was 0-18% that of controls. When NE content was 15-23% of normal, MAP increased 49-62% regardless of the denervation technique.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1990

Volume

259

Issue

5 Pt 1

Start / End Page

G781 / G785

Location

United States

Related Subject Headings

  • Rats, Inbred Lew
  • Rats
  • Phenols
  • Phenol
  • Norepinephrine
  • Microsurgery
  • Methods
  • Male
  • Liver Transplantation
  • Liver
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cucchiaro, G., Yamaguchi, Y., Mills, E., Kuhn, C. M., Anthony, D. C., Branum, G. D., … Meyers, W. C. (1990). Evaluation of selective liver denervation methods. Am J Physiol, 259(5 Pt 1), G781–G785. https://doi.org/10.1152/ajpgi.1990.259.5.G781
Cucchiaro, G., Y. Yamaguchi, E. Mills, C. M. Kuhn, D. C. Anthony, G. D. Branum, R. Epstein, and W. C. Meyers. “Evaluation of selective liver denervation methods.Am J Physiol 259, no. 5 Pt 1 (November 1990): G781–85. https://doi.org/10.1152/ajpgi.1990.259.5.G781.
Cucchiaro G, Yamaguchi Y, Mills E, Kuhn CM, Anthony DC, Branum GD, et al. Evaluation of selective liver denervation methods. Am J Physiol. 1990 Nov;259(5 Pt 1):G781–5.
Cucchiaro, G., et al. “Evaluation of selective liver denervation methods.Am J Physiol, vol. 259, no. 5 Pt 1, Nov. 1990, pp. G781–85. Pubmed, doi:10.1152/ajpgi.1990.259.5.G781.
Cucchiaro G, Yamaguchi Y, Mills E, Kuhn CM, Anthony DC, Branum GD, Epstein R, Meyers WC. Evaluation of selective liver denervation methods. Am J Physiol. 1990 Nov;259(5 Pt 1):G781–G785.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1990

Volume

259

Issue

5 Pt 1

Start / End Page

G781 / G785

Location

United States

Related Subject Headings

  • Rats, Inbred Lew
  • Rats
  • Phenols
  • Phenol
  • Norepinephrine
  • Microsurgery
  • Methods
  • Male
  • Liver Transplantation
  • Liver