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A novel dwarfing mutation in a green revolution gene from Brassica rapa.

Publication ,  Journal Article
Muangprom, A; Thomas, SG; Sun, T-P; Osborn, TC
Published in: Plant physiology
March 2005

Mutations in the biosynthesis or signaling pathways of gibberellin (GA) can cause dwarfing phenotypes in plants, and the use of such mutations in plant breeding was a major factor in the success of the Green Revolution. DELLA proteins are GA signaling repressors whose functions are conserved in different plant species. Recent studies show that GA promotes stem growth by causing degradation of DELLA proteins via the ubiquitin-proteasome pathway. The most widely utilized dwarfing alleles in wheat (Triticum aestivum; e.g. Rht-B1b and Rht-D1b) encode GA-resistant forms of a DELLA protein that function as dominant and constitutively active repressors of stem growth. All of the previously identified dominant DELLA repressors from several plant species contain N-terminal mutations. Here we report on a novel dwarf mutant from Brassica rapa (Brrga1-d) that is caused by substitution of a conserved amino acid in the C-terminal domain of a DELLA protein. Brrga1-d, like N-terminal DELLA mutants, retains its repressor function and accumulates to high levels, even in the presence of GA. However, unlike wild-type and N-terminal DELLA mutants, Brrga1-d does not interact with a protein component required for degradation, suggesting that the mutated amino acid causes dwarfism by preventing an interaction needed for its degradation. This novel mutation confers nondeleterious dwarf phenotypes when transferred to Arabidopsis (Arabidopsis thaliana) and oilseed rape (Brassica napus), indicating its potential usefulness in other crop species.

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Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

March 2005

Volume

137

Issue

3

Start / End Page

931 / 938

Related Subject Headings

  • Two-Hybrid System Techniques
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae
  • Plant Proteins
  • Plant Biology & Botany
  • Phenotype
  • Mutation
  • Molecular Sequence Data
  • Gibberellins
 

Citation

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Muangprom, A., Thomas, S. G., Sun, T.-P., & Osborn, T. C. (2005). A novel dwarfing mutation in a green revolution gene from Brassica rapa. Plant Physiology, 137(3), 931–938. https://doi.org/10.1104/pp.104.057646
Muangprom, Amorntip, Stephen G. Thomas, Tai-Ping Sun, and Thomas C. Osborn. “A novel dwarfing mutation in a green revolution gene from Brassica rapa.Plant Physiology 137, no. 3 (March 2005): 931–38. https://doi.org/10.1104/pp.104.057646.
Muangprom A, Thomas SG, Sun T-P, Osborn TC. A novel dwarfing mutation in a green revolution gene from Brassica rapa. Plant physiology. 2005 Mar;137(3):931–8.
Muangprom, Amorntip, et al. “A novel dwarfing mutation in a green revolution gene from Brassica rapa.Plant Physiology, vol. 137, no. 3, Mar. 2005, pp. 931–38. Epmc, doi:10.1104/pp.104.057646.
Muangprom A, Thomas SG, Sun T-P, Osborn TC. A novel dwarfing mutation in a green revolution gene from Brassica rapa. Plant physiology. 2005 Mar;137(3):931–938.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

March 2005

Volume

137

Issue

3

Start / End Page

931 / 938

Related Subject Headings

  • Two-Hybrid System Techniques
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae
  • Plant Proteins
  • Plant Biology & Botany
  • Phenotype
  • Mutation
  • Molecular Sequence Data
  • Gibberellins