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Measurement of cell migration on surface-bound fibronectin gradients.

Publication ,  Journal Article
Smith, JT; Tomfohr, JK; Wells, MC; Beebe, TP; Kepler, TB; Reichert, WM
Published in: Langmuir
September 14, 2004

A novel technique for the quantitative observation of cell migration along linear gradient substrates functionalized with adhesive proteins is presented. Gradients of the cell adhesion molecule fibronectin are generated by the cross diffusion of functionalizable alkanethiols on gold and characterized by X-ray photoelectron spectroscopy and surface plasmon resonance. Two distinct migration assays are described that characterize the movement of either sparsely populated noncontacting cells or a confluent monolayer of cells into free space. The drift speed of bovine aortic endothelial cells is measured and shown to increase along a fibronectin gradient when compared to a uniform control substrate using both assays. The results of these experiments establish reproducible conditions for studies of cell migration on gradients of surface-bound ligands.

Duke Scholars

Published In

Langmuir

DOI

ISSN

0743-7463

Publication Date

September 14, 2004

Volume

20

Issue

19

Start / End Page

8279 / 8286

Location

United States

Related Subject Headings

  • Surface Properties
  • Surface Plasmon Resonance
  • Spectrometry, X-Ray Emission
  • Pilot Projects
  • Fibronectins
  • Endothelial Cells
  • Chemical Physics
  • Cells, Cultured
  • Cell Movement
  • Cattle
 

Citation

APA
Chicago
ICMJE
MLA
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Smith, J. T., Tomfohr, J. K., Wells, M. C., Beebe, T. P., Kepler, T. B., & Reichert, W. M. (2004). Measurement of cell migration on surface-bound fibronectin gradients. Langmuir, 20(19), 8279–8286. https://doi.org/10.1021/la0489763
Smith, Jason T., John K. Tomfohr, Matthew C. Wells, Thomas P. Beebe, Thomas B. Kepler, and W Monty Reichert. “Measurement of cell migration on surface-bound fibronectin gradients.Langmuir 20, no. 19 (September 14, 2004): 8279–86. https://doi.org/10.1021/la0489763.
Smith JT, Tomfohr JK, Wells MC, Beebe TP, Kepler TB, Reichert WM. Measurement of cell migration on surface-bound fibronectin gradients. Langmuir. 2004 Sep 14;20(19):8279–86.
Smith, Jason T., et al. “Measurement of cell migration on surface-bound fibronectin gradients.Langmuir, vol. 20, no. 19, Sept. 2004, pp. 8279–86. Pubmed, doi:10.1021/la0489763.
Smith JT, Tomfohr JK, Wells MC, Beebe TP, Kepler TB, Reichert WM. Measurement of cell migration on surface-bound fibronectin gradients. Langmuir. 2004 Sep 14;20(19):8279–8286.
Journal cover image

Published In

Langmuir

DOI

ISSN

0743-7463

Publication Date

September 14, 2004

Volume

20

Issue

19

Start / End Page

8279 / 8286

Location

United States

Related Subject Headings

  • Surface Properties
  • Surface Plasmon Resonance
  • Spectrometry, X-Ray Emission
  • Pilot Projects
  • Fibronectins
  • Endothelial Cells
  • Chemical Physics
  • Cells, Cultured
  • Cell Movement
  • Cattle