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Local and differential control of vegetative storage protein expression in response to herbivore damage in Arabidopsis thaliana.

Publication ,  Journal Article
Berger, S; Mitchell-Olds, T; Stotz, HU
Published in: Physiologia plantarum
January 2002

Vegetative storage proteins (VSPs) are thought to fulfil important nutritional roles during plant development and stress adaptation. Plant responses to mechanical wounding and herbivore damage include an activation of VSP expression. It was recently suggested that vsp is part of the systemic response of Arabidopsis to wounding. To test this proposal, we monitored the spatial regulation of vsp mRNAs and VSP proteins. Arabidopsis contains two vsp genes and real-time quantitative PCR allowed us to characterize their differential expression. The ratio of vsp1 to vsp2 mRNA abundance increased when plants were challenged with diamondback moth larvae or Egyptian cotton worms, but not when they were mechanically wounded. We observed a dramatic increase of vsp1 and vsp2 mRNA as well as VSP protein levels in leaves that experienced herbivore damage. By contrast, there was a relatively minor increase of vsp mRNA and VSP protein levels in undamaged leaves of infested plants. These results clearly demonstrate that VSPs are part of the local plant response to herbivore attack. To obtain additional information on vsp regulation, we analysed a fusion of a soybean vspB promoter fragment to the beta-glucuronidase gene in transgenic Arabidopsis plants. The vspB promoter responded to both jasmonate and herbivore treatments, suggesting that similar signals regulate its expression in both plant species.

Duke Scholars

Published In

Physiologia plantarum

DOI

EISSN

1399-3054

ISSN

1399-3054

Publication Date

January 2002

Volume

114

Issue

1

Start / End Page

85 / 91

Related Subject Headings

  • Plant Biology & Botany
  • 3108 Plant biology
  • 3008 Horticultural production
  • 0706 Horticultural Production
  • 0607 Plant Biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Berger, S., Mitchell-Olds, T., & Stotz, H. U. (2002). Local and differential control of vegetative storage protein expression in response to herbivore damage in Arabidopsis thaliana. Physiologia Plantarum, 114(1), 85–91. https://doi.org/10.1046/j.0031-9317.2001.1140112.x
Berger, Susanne, Thomas Mitchell-Olds, and Henrik U. Stotz. “Local and differential control of vegetative storage protein expression in response to herbivore damage in Arabidopsis thaliana.Physiologia Plantarum 114, no. 1 (January 2002): 85–91. https://doi.org/10.1046/j.0031-9317.2001.1140112.x.
Berger, Susanne, et al. “Local and differential control of vegetative storage protein expression in response to herbivore damage in Arabidopsis thaliana.Physiologia Plantarum, vol. 114, no. 1, Jan. 2002, pp. 85–91. Epmc, doi:10.1046/j.0031-9317.2001.1140112.x.
Journal cover image

Published In

Physiologia plantarum

DOI

EISSN

1399-3054

ISSN

1399-3054

Publication Date

January 2002

Volume

114

Issue

1

Start / End Page

85 / 91

Related Subject Headings

  • Plant Biology & Botany
  • 3108 Plant biology
  • 3008 Horticultural production
  • 0706 Horticultural Production
  • 0607 Plant Biology
  • 0601 Biochemistry and Cell Biology