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Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD.

Publication ,  Journal Article
Xu, J; Luo, G; Vilgalys, RJ; Brandt, ME; Mitchell, TG
Published in: Microbiology (Reading)
January 2002

Cryptococcus neoformans is a major pathogen of humans throughout the world. Using commercial mAbs to capsular epitopes, strains of C. neoformans manifest five distinct serotypes--A, B, C, D and AD. Previous studies demonstrated significant divergence among serotypes A, B, C and D, which are thought to be haploid. In this study the origins and evolution of strains of serotype AD were investigated. A portion (537 bp) of the laccase gene was cloned and sequenced from 14 strains of serotype AD. Each strain contained two different alleles and sequences for both alleles were obtained. These sequences were compared to those from serotypes A, B, C and D. This analysis indicated that each of the 14 serotype AD strains contained two phylogenetically distinct haplotypes: one haplotype was highly similar to the serotype A group and the other to the serotype D group. To explain the origins of these serotype AD strains, genealogical analysis is consistent with at least three recent and independent hybridization events. The results demonstrate that the evolution of C. neoformans is continuing and dynamic.

Duke Scholars

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

January 2002

Volume

148

Issue

Pt 1

Start / End Page

203 / 212

Location

England

Related Subject Headings

  • Serotyping
  • Sequence Analysis, DNA
  • Phylogeny
  • Oxidoreductases
  • Molecular Sequence Data
  • Microbiology
  • Laccase
  • Humans
  • Genetic Variation
  • Evolution, Molecular
 

Citation

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Xu, J., Luo, G., Vilgalys, R. J., Brandt, M. E., & Mitchell, T. G. (2002). Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD. Microbiology (Reading), 148(Pt 1), 203–212. https://doi.org/10.1099/00221287-148-1-203
Xu, Jianping, Guizhen Luo, Rytas J. Vilgalys, Mary E. Brandt, and Thomas G. Mitchell. “Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD.Microbiology (Reading) 148, no. Pt 1 (January 2002): 203–12. https://doi.org/10.1099/00221287-148-1-203.
Xu J, Luo G, Vilgalys RJ, Brandt ME, Mitchell TG. Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD. Microbiology (Reading). 2002 Jan;148(Pt 1):203–12.
Xu, Jianping, et al. “Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD.Microbiology (Reading), vol. 148, no. Pt 1, Jan. 2002, pp. 203–12. Pubmed, doi:10.1099/00221287-148-1-203.
Xu J, Luo G, Vilgalys RJ, Brandt ME, Mitchell TG. Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD. Microbiology (Reading). 2002 Jan;148(Pt 1):203–212.

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

January 2002

Volume

148

Issue

Pt 1

Start / End Page

203 / 212

Location

England

Related Subject Headings

  • Serotyping
  • Sequence Analysis, DNA
  • Phylogeny
  • Oxidoreductases
  • Molecular Sequence Data
  • Microbiology
  • Laccase
  • Humans
  • Genetic Variation
  • Evolution, Molecular