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[Genetic structure of geographically different populations of candida albicans].

Publication ,  Journal Article
Schönian, G; Forche, A; Tietz, HJ; Müller, M; Gräser, Y; Vilgalys, R; Mitchell, TG; Presber, W
Published in: Mycoses
2000

Codominant single-locus markers were developed by amplifying genomic DNA of C. albicans with pairs of random primers. Monomorphic PCR products were screened for polymorphisms by the SSCP technique. Sequencing confirmed that SSCP's were mostly due to single nucleotide substitutions in the polymorphic fragments. A total of 85 polymorphic loci were observed within 13 PCR fragments. Populations from Africa displayed less genotype variation than the populations from Europe and USA. Two genetically similar African C. albicans populations exhibiting an atypical biotype were strictly clonal and perhaps represent a geographically distributed clone. Analyses of "typical" C. albicans populations of different geographical origin provided however evidence for both clonality and recombination. Evidence for clonality was supported by the absence of segregation genotypes, and by deviation of genotypic frequencies from Hardy-Weinberg expectations. Tests for nonrandom association of alleles across loci revealed less evidence for linkage disequilibrium than expected for strictly clonal populations. Although all C. albicans populations tested were primarily clonal, evidence for recombination suggests that sexual reproduction or some other form of genetic exchange occurs in this species.

Duke Scholars

Published In

Mycoses

ISSN

0933-7407

Publication Date

2000

Volume

43 Suppl 2

Start / End Page

51 / 56

Location

Germany

Related Subject Headings

  • United States
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Microbiology
  • Genotype
  • Genetic Variation
  • Europe
  • DNA, Fungal
  • Candida albicans
  • Africa
 

Citation

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Schönian, G., Forche, A., Tietz, H. J., Müller, M., Gräser, Y., Vilgalys, R., … Presber, W. (2000). [Genetic structure of geographically different populations of candida albicans]. Mycoses, 43 Suppl 2, 51–56.
Schönian, G., A. Forche, H. J. Tietz, M. Müller, Y. Gräser, R. Vilgalys, T. G. Mitchell, and W. Presber. “[Genetic structure of geographically different populations of candida albicans].Mycoses 43 Suppl 2 (2000): 51–56.
Schönian G, Forche A, Tietz HJ, Müller M, Gräser Y, Vilgalys R, et al. [Genetic structure of geographically different populations of candida albicans]. Mycoses. 2000;43 Suppl 2:51–6.
Schönian, G., et al. “[Genetic structure of geographically different populations of candida albicans].Mycoses, vol. 43 Suppl 2, 2000, pp. 51–56.
Schönian G, Forche A, Tietz HJ, Müller M, Gräser Y, Vilgalys R, Mitchell TG, Presber W. [Genetic structure of geographically different populations of candida albicans]. Mycoses. 2000;43 Suppl 2:51–56.
Journal cover image

Published In

Mycoses

ISSN

0933-7407

Publication Date

2000

Volume

43 Suppl 2

Start / End Page

51 / 56

Location

Germany

Related Subject Headings

  • United States
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Microbiology
  • Genotype
  • Genetic Variation
  • Europe
  • DNA, Fungal
  • Candida albicans
  • Africa