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Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34.

Publication ,  Journal Article
Storms, RW; Green, PD; Safford, KM; Niedzwiecki, D; Cogle, CR; Colvin, OM; Chao, NJ; Rice, HE; Smith, CA
Published in: Blood
July 1, 2005

A broad range of hematopoietic stem cells and progenitors reside within a fraction of umbilical cord blood (UCB) that exhibits low light scatter properties (SSC(lo)) and high expression of aldehyde dehydrogenase (ALDH(br)). Many SSC(lo) ALDH(br) cells coexpress CD34; however, other cells express either ALDH or CD34. To investigate the developmental potential of these cell subsets, purified ALDH(br) CD34+, ALDH(neg) CD34+, and ALDH(br) CD34(neg) UCB cells were characterized within a variety of in vivo and in vitro assays. Primitive progenitors capable of multilineage development were monitored in long- and short-term repopulation assays performed on nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, and in primary and secondary long-term culture assays. These progenitors were highly enriched within the ALDH(br) CD34+ fraction. This cell fraction also enriched short-term myeloid progenitors that were detected in vitro. By comparison, ALDH(neg) CD34+ cells contained few primitive progenitors and had diminished short-term myeloid potential but exhibited enhanced short-term natural killer (NK) cell development in vitro. The ALDH(br) CD34(neg) cells were not efficiently supported by any of the assays used. These studies suggested that in particular the expression of ALDH delineated distinct CD34+ stem cell and progenitor compartments. The differential expression of ALDH may provide a means to explore normal and malignant processes associated with myeloid and lymphoid development.

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Published In

Blood

DOI

ISSN

0006-4971

Publication Date

July 1, 2005

Volume

106

Issue

1

Start / End Page

95 / 102

Location

United States

Related Subject Headings

  • Myeloid Cells
  • Multipotent Stem Cells
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Lymphocytes
  • Killer Cells, Natural
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Storms, R. W., Green, P. D., Safford, K. M., Niedzwiecki, D., Cogle, C. R., Colvin, O. M., … Smith, C. A. (2005). Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34. Blood, 106(1), 95–102. https://doi.org/10.1182/blood-2004-09-3652
Storms, Robert W., Patrick D. Green, Kristine M. Safford, Donna Niedzwiecki, Christopher R. Cogle, O Michael Colvin, Nelson J. Chao, Henry E. Rice, and Clayton A. Smith. “Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34.Blood 106, no. 1 (July 1, 2005): 95–102. https://doi.org/10.1182/blood-2004-09-3652.
Storms RW, Green PD, Safford KM, Niedzwiecki D, Cogle CR, Colvin OM, et al. Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34. Blood. 2005 Jul 1;106(1):95–102.
Storms, Robert W., et al. “Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34.Blood, vol. 106, no. 1, July 2005, pp. 95–102. Pubmed, doi:10.1182/blood-2004-09-3652.
Storms RW, Green PD, Safford KM, Niedzwiecki D, Cogle CR, Colvin OM, Chao NJ, Rice HE, Smith CA. Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34. Blood. 2005 Jul 1;106(1):95–102.

Published In

Blood

DOI

ISSN

0006-4971

Publication Date

July 1, 2005

Volume

106

Issue

1

Start / End Page

95 / 102

Location

United States

Related Subject Headings

  • Myeloid Cells
  • Multipotent Stem Cells
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Lymphocytes
  • Killer Cells, Natural
  • Immunology
  • Humans
  • Hematopoietic Stem Cells