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Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence.

Publication ,  Journal Article
Mutzner, PA; Stuhlmiller, GM; Seigler, HF
Published in: J Surg Oncol
1980

Xenoantiserum, alloantiserum, and autoantiserum prepared against human melanoma cells from cell lines maintained in tissue culture were evaluated by indirect immunofluorescence (ind IF) for their content of antibodies reactive with melanoma cell membrane tumor-associated antigens (TAA). Following absorptions which removed all detectable anti-human species, anti-RBC, and anti-HLA antigen activity, the antisera were tested against a panel of human cell lines. Three apparently distinct melanoma-associated cell membrane antigens were detected. The first of these was present on all human cells maintained in tissue culture including cells of both malignant and nonmalignant origin. The second antigen was expressed on all cells of malignant origin and also by fetal fibroblasts. The third antigen was detectable only on melanoma cells and was expressed by a majority of the melanomas tested. No evidence was obtained to suggest the presence of melanoma TAA unique to a single melanoma cell line. Marked differences were observed in the antigenic profile detected by the three antisera.

Duke Scholars

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

1980

Volume

14

Issue

4

Start / End Page

367 / 377

Location

United States

Related Subject Headings

  • Pan troglodytes
  • Oncology & Carcinogenesis
  • Melanoma
  • Immune Sera
  • Humans
  • Fluorescent Antibody Technique
  • Fibroblasts
  • Fetus
  • Cytotoxicity Tests, Immunologic
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mutzner, P. A., Stuhlmiller, G. M., & Seigler, H. F. (1980). Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence. J Surg Oncol, 14(4), 367–377. https://doi.org/10.1002/jso.2930140411
Mutzner, P. A., G. M. Stuhlmiller, and H. F. Seigler. “Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence.J Surg Oncol 14, no. 4 (1980): 367–77. https://doi.org/10.1002/jso.2930140411.
Mutzner, P. A., et al. “Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence.J Surg Oncol, vol. 14, no. 4, 1980, pp. 367–77. Pubmed, doi:10.1002/jso.2930140411.
Journal cover image

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

1980

Volume

14

Issue

4

Start / End Page

367 / 377

Location

United States

Related Subject Headings

  • Pan troglodytes
  • Oncology & Carcinogenesis
  • Melanoma
  • Immune Sera
  • Humans
  • Fluorescent Antibody Technique
  • Fibroblasts
  • Fetus
  • Cytotoxicity Tests, Immunologic
  • Cells, Cultured