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Melanoma-specific cytotoxic T cells generated from peripheral blood lymphocytes. Implications of a renewable source of precursors for adoptive cellular immunotherapy.

Publication ,  Journal Article
Slingluff, CL; Darrow, TL; Seigler, HF
Published in: Ann Surg
August 1989

Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been generated in vitro from peripheral blood lymphocytes (PBL) of five patients with resectable stage II malignant melanoma. The PBL were cultured with 5u/ml recombinant IL-2 and were repeatedly stimulated with irradiated fresh or cultured autologous tumor cells. Cytotoxicity was determined by four-hour chromium release assays. Specific cytotoxicity developed in 30 to 40 days, after three or four stimulations with tumor. The PBL-derived CTL are CD3+ and are mixed for CD4+ and CD8+ phenotypes. They lysed autologous melanoma and failed to lyse allogeneic melanoma, K562, or autologous lymphocytes. The lysis of autologous tumor was maintained for more than 4 months. The cells proliferated in response to autologous, but not allogeneic melanoma cells, in a dose-dependent manner. Lysis of the autologous tumor target was inhibited with w6/32, a monoclonal antibody to HLA Class I antigens. It is concluded that PBL may serve as a plentiful and renewable source of precursor cells for the generation of autologous tumor-specific CTL, which may be useful in specific adoptive cellular immunotherapy of melanoma.

Duke Scholars

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Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

August 1989

Volume

210

Issue

2

Start / End Page

194 / 202

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Recombinant Proteins
  • Melanoma
  • Lymphocytes
  • Lymphocyte Activation
  • Interleukin-2
  • Immunotherapy
  • Humans
  • Histocompatibility Antigens Class I
 

Citation

APA
Chicago
ICMJE
MLA
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Slingluff, C. L., Darrow, T. L., & Seigler, H. F. (1989). Melanoma-specific cytotoxic T cells generated from peripheral blood lymphocytes. Implications of a renewable source of precursors for adoptive cellular immunotherapy. Ann Surg, 210(2), 194–202. https://doi.org/10.1097/00000658-198908000-00010
Slingluff, C. L., T. L. Darrow, and H. F. Seigler. “Melanoma-specific cytotoxic T cells generated from peripheral blood lymphocytes. Implications of a renewable source of precursors for adoptive cellular immunotherapy.Ann Surg 210, no. 2 (August 1989): 194–202. https://doi.org/10.1097/00000658-198908000-00010.
Slingluff, C. L., et al. “Melanoma-specific cytotoxic T cells generated from peripheral blood lymphocytes. Implications of a renewable source of precursors for adoptive cellular immunotherapy.Ann Surg, vol. 210, no. 2, Aug. 1989, pp. 194–202. Pubmed, doi:10.1097/00000658-198908000-00010.

Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

August 1989

Volume

210

Issue

2

Start / End Page

194 / 202

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Recombinant Proteins
  • Melanoma
  • Lymphocytes
  • Lymphocyte Activation
  • Interleukin-2
  • Immunotherapy
  • Humans
  • Histocompatibility Antigens Class I