Scholars@Duke publication: Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages.

Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages.

Publication , Journal Article

Guo, H; Wei, J; Kuo, PC

Published in: Biochem Biophys Res Commun

December 21, 2001

In LPS-mediated states of sepsis, inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production inhibit cellular respiration and mitochondrial electron transport. NO has been demonstrated to inhibit mitochondrial respiration by nitrosylation of the iron-sulfur centers of aconitase, complex I (NADH-ubiquinone oxidoreductase), complex II (succinate-ubiquinone oxidoreductase), and complex IV (cytochrome c oxidase). However, little is known of the effect of NO on expression of critical proteins in the electron transport chain. In ANA-1 murine macrophages, LPS-mediated NO synthesis decreases Cyt b protein expression and steady-state mRNA levels. Mitochondrial run-on analysis demonstrates unaltered Cyt b mitochondrial gene transcription. In this study utilizing LPS-stimulated ANA-1 murine macrophages, we demonstrate that expression of the mitochondrial protein, Cyt b, is significantly decreased as the result of a unique and previously unknown, NO-dependent posttranscriptional regulatory mechanism. (c)2001 Elsevier Science.

Duke Scholars

Author Hongtao Michael Guo Orthopaedic Surgery

Author Paul C. Kuo Surgery

Published In

Biochem Biophys Res Commun

DOI

10.1006/bbrc.2001.6107

ISSN

0006-291X

Publication Date

December 21, 2001

Volume

289

Issue

Start / End Page

993 / 997

Location

United States

Related Subject Headings

Transcription, Genetic
RNA, Messenger
Nitric Oxide
Mitochondria
Mice
Macrophages
Lipopolysaccharides
Gene Expression
Electron Transport
Dose-Response Relationship, Drug

Citation

APA

Chicago

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MLA

NLM

Guo, H., Wei, J., & Kuo, P. C. (2001). Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages. Biochem Biophys Res Commun, 289(5), 993–997. https://doi.org/10.1006/bbrc.2001.6107

Guo, H., J. Wei, and P. C. Kuo. “Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages.” Biochem Biophys Res Commun 289, no. 5 (December 21, 2001): 993–97. https://doi.org/10.1006/bbrc.2001.6107.

Guo H, Wei J, Kuo PC. Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages. Biochem Biophys Res Commun. 2001 Dec 21;289(5):993–7.

Guo, H., et al. “Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages.” Biochem Biophys Res Commun, vol. 289, no. 5, Dec. 2001, pp. 993–97. Pubmed, doi:10.1006/bbrc.2001.6107.

Guo H, Wei J, Kuo PC. Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages. Biochem Biophys Res Commun. 2001 Dec 21;289(5):993–997.