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Relationship between histopathology and in vitro clonogenicity in breast cancer.

Publication ,  Journal Article
Moezzi, J; Ali-Osman, F; Nicholson, GL; Ungerleider, JS; Murphy, MJ
Published in: Breast Cancer Res Treat
1986

The human tumor clonogenic assay was used to culture 268 primary and metastatic breast cancer samples. Cultures of 181 specimens were prepared in the double-layer agar system and 87 in a modified system utilizing a liquid upper layer. Successful growth (greater than 5 colonies) was 53% for the agar 2-layer method and 68% for the modified system. Three morphologically distinct colony types were observed: Type I--small, dark, compact colonies; Type II--clear colonies; and Type III--mixed colonies of Types I and II. In 73 cases the histologic slides of the original tumor specimens were reviewed, and the histopathological findings correlated with the clonogenicity of the specimens. There was a significant positive relationship between the degree of differentiation and focal microscopic tumor necrosis in the original specimen and its subsequent clonogenicity. However, other histologic parameters did not show any relationship with clonogenicity.

Duke Scholars

Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

1986

Volume

8

Issue

2

Start / End Page

147 / 156

Location

Netherlands

Related Subject Headings

  • Tumor Stem Cell Assay
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Necrosis
  • Humans
  • Fibrosis
  • Female
  • Cell Aggregation
  • Breast Neoplasms
  • 1112 Oncology and Carcinogenesis
 

Citation

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Moezzi, J., Ali-Osman, F., Nicholson, G. L., Ungerleider, J. S., & Murphy, M. J. (1986). Relationship between histopathology and in vitro clonogenicity in breast cancer. Breast Cancer Res Treat, 8(2), 147–156. https://doi.org/10.1007/BF01807703
Moezzi, J., F. Ali-Osman, G. L. Nicholson, J. S. Ungerleider, and M. J. Murphy. “Relationship between histopathology and in vitro clonogenicity in breast cancer.Breast Cancer Res Treat 8, no. 2 (1986): 147–56. https://doi.org/10.1007/BF01807703.
Moezzi J, Ali-Osman F, Nicholson GL, Ungerleider JS, Murphy MJ. Relationship between histopathology and in vitro clonogenicity in breast cancer. Breast Cancer Res Treat. 1986;8(2):147–56.
Moezzi, J., et al. “Relationship between histopathology and in vitro clonogenicity in breast cancer.Breast Cancer Res Treat, vol. 8, no. 2, 1986, pp. 147–56. Pubmed, doi:10.1007/BF01807703.
Moezzi J, Ali-Osman F, Nicholson GL, Ungerleider JS, Murphy MJ. Relationship between histopathology and in vitro clonogenicity in breast cancer. Breast Cancer Res Treat. 1986;8(2):147–156.
Journal cover image

Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

1986

Volume

8

Issue

2

Start / End Page

147 / 156

Location

Netherlands

Related Subject Headings

  • Tumor Stem Cell Assay
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Necrosis
  • Humans
  • Fibrosis
  • Female
  • Cell Aggregation
  • Breast Neoplasms
  • 1112 Oncology and Carcinogenesis