Rapid method for permanent slide preparation of colonies in soft agar cultures.
Publication
, Journal Article
Moezzi, J; Ali-Osman, F; Murphy, MJ
Published in: Int J Cell Cloning
September 1986
A method is presented for preparing permanent microscopic slides from colony-bearing agar layers in soft agar cultures. The main advantages of this technique are its simplicity, rapidity and accurate colony preservation. This method could have broad applications in the human tumor clonogenic assay (HTCA), particularly in the quantitative morphological, cytochemical and immunocytochemical assessment of colonies that form in both control and drug-treated cultures. Thus, this method opens up possibilities for using cytopathological criteria as a quantitative endpoint of the HTCA.
Duke Scholars
Published In
Int J Cell Cloning
DOI
ISSN
0737-1454
Publication Date
September 1986
Volume
4
Issue
5
Start / End Page
368 / 374
Location
United States
Related Subject Headings
- Humans
- Histological Techniques
- Culture Techniques
- Clone Cells
- Cells, Cultured
- Agar
Citation
APA
Chicago
ICMJE
MLA
NLM
Moezzi, J., Ali-Osman, F., & Murphy, M. J. (1986). Rapid method for permanent slide preparation of colonies in soft agar cultures. Int J Cell Cloning, 4(5), 368–374. https://doi.org/10.1002/stem.5530040508
Moezzi, J., F. Ali-Osman, and M. J. Murphy. “Rapid method for permanent slide preparation of colonies in soft agar cultures.” Int J Cell Cloning 4, no. 5 (September 1986): 368–74. https://doi.org/10.1002/stem.5530040508.
Moezzi J, Ali-Osman F, Murphy MJ. Rapid method for permanent slide preparation of colonies in soft agar cultures. Int J Cell Cloning. 1986 Sep;4(5):368–74.
Moezzi, J., et al. “Rapid method for permanent slide preparation of colonies in soft agar cultures.” Int J Cell Cloning, vol. 4, no. 5, Sept. 1986, pp. 368–74. Pubmed, doi:10.1002/stem.5530040508.
Moezzi J, Ali-Osman F, Murphy MJ. Rapid method for permanent slide preparation of colonies in soft agar cultures. Int J Cell Cloning. 1986 Sep;4(5):368–374.
Published In
Int J Cell Cloning
DOI
ISSN
0737-1454
Publication Date
September 1986
Volume
4
Issue
5
Start / End Page
368 / 374
Location
United States
Related Subject Headings
- Humans
- Histological Techniques
- Culture Techniques
- Clone Cells
- Cells, Cultured
- Agar