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Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers.

Publication ,  Journal Article
Norris, J; Fan, D; Aleman, C; Marks, JR; Futreal, PA; Wiseman, RW; Iglehart, JD; Deininger, PL; McDonnell, DP
Published in: J Biol Chem
September 29, 1995

We have utilized a genetic selection system in yeast to identify novel estrogen-responsive genes within the human genome and to define the sequences in the BRCA-1 gene responsible for its estrogen responsiveness. This approach led to the identification of a new subclass within the Alu family of DNA repeats which have diverged from known Alu sequences and have acquired the ability to function as estrogen receptor-dependent enhancers. Importantly, these new elements confer receptor-dependent estrogen responsiveness to a heterologous promoter when assayed in mammalian cells. This transcriptional activity can be attenuated by the addition of either of three different classes of estrogen receptor antagonists, indicating that these elements function as classical estrogen receptor-dependent enhancers. Furthermore, this enhancer activity is restricted to a specific subset of DNA repeats because consensus Alu elements of four major subfamilies do not respond to the estrogen receptor. Previously, most Alu sequences have been considered to be functionally inert. However, this work provides strong evidence that a significant subset can confer estrogen responsiveness upon a promoter within which they are located. Clearly, Alu sequences must now be considered as important contributors to the regulation of gene transcription in estrogen receptor-containing cells.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 29, 1995

Volume

270

Issue

39

Start / End Page

22777 / 22782

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Repetitive Sequences, Nucleic Acid
  • Receptors, Estrogen
  • Promoter Regions, Genetic
  • Neoplasm Proteins
  • Molecular Sequence Data
 

Citation

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Norris, J., Fan, D., Aleman, C., Marks, J. R., Futreal, P. A., Wiseman, R. W., … McDonnell, D. P. (1995). Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers. J Biol Chem, 270(39), 22777–22782. https://doi.org/10.1074/jbc.270.39.22777
Norris, J., D. Fan, C. Aleman, J. R. Marks, P. A. Futreal, R. W. Wiseman, J. D. Iglehart, P. L. Deininger, and D. P. McDonnell. “Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers.J Biol Chem 270, no. 39 (September 29, 1995): 22777–82. https://doi.org/10.1074/jbc.270.39.22777.
Norris J, Fan D, Aleman C, Marks JR, Futreal PA, Wiseman RW, et al. Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers. J Biol Chem. 1995 Sep 29;270(39):22777–82.
Norris, J., et al. “Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers.J Biol Chem, vol. 270, no. 39, Sept. 1995, pp. 22777–82. Pubmed, doi:10.1074/jbc.270.39.22777.
Norris J, Fan D, Aleman C, Marks JR, Futreal PA, Wiseman RW, Iglehart JD, Deininger PL, McDonnell DP. Identification of a new subclass of Alu DNA repeats which can function as estrogen receptor-dependent transcriptional enhancers. J Biol Chem. 1995 Sep 29;270(39):22777–22782.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 29, 1995

Volume

270

Issue

39

Start / End Page

22777 / 22782

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Repetitive Sequences, Nucleic Acid
  • Receptors, Estrogen
  • Promoter Regions, Genetic
  • Neoplasm Proteins
  • Molecular Sequence Data