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Calcitonin gene-related peptide and reperfusion injury.

Publication ,  Journal Article
Allen, DM; Chen, LE; Seaber, AV; Urbaniak, JR
Published in: J Orthop Res
March 1997

Calcitonin gene-related peptide is a potent intrinsic vasodilator, can induce prostacyclin release, and may inhibit membrane lipid peroxidation. This study examines the effect of calcitonin gene-related peptide on vessel diameters, capillary perfusion, and contractile function of skeletal muscle after 4 or 5 hours of ischemia and during immediate reperfusion using the rat cremaster muscle model. Forty-two male rats were used; half of these received 0.2 ml of 10(-7) M calcitonin gene-related peptide after 0, 15, and 30 minutes of reperfusion, while the other half received normal saline as a control. By means of intravital videomicroscopy, the diameters of 10 vessels per muscle were measured prior to ischemia and during reperfusion. The fluorescein filling area was determined at 15, 30, and 60 minutes of reperfusion. After 1 hour of reperfusion, muscle function was examined in vitro by quantifying the contractile response to electric field stimulation of the muscles in an organ bath system. There was a significant increase in the diameter of the arterioles, but not the small arteries, at every time point from 10 to 60 minutes of reperfusion. The fluorescein filling area was increased in treated muscles at every time point. Contractile function was not significantly preserved. In light of the ability of calcitonin gene-related peptide to relieve vasospasm and improve capillary perfusion, it may be useful in reducing reperfusion injury in the future.

Duke Scholars

Published In

J Orthop Res

DOI

ISSN

0736-0266

Publication Date

March 1997

Volume

15

Issue

2

Start / End Page

243 / 248

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Time Factors
  • Reperfusion Injury
  • Rats, Sprague-Dawley
  • Rats
  • Orthopedics
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Ischemia
 

Citation

APA
Chicago
ICMJE
MLA
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Allen, D. M., Chen, L. E., Seaber, A. V., & Urbaniak, J. R. (1997). Calcitonin gene-related peptide and reperfusion injury. J Orthop Res, 15(2), 243–248. https://doi.org/10.1002/jor.1100150213
Allen, D. M., L. E. Chen, A. V. Seaber, and J. R. Urbaniak. “Calcitonin gene-related peptide and reperfusion injury.J Orthop Res 15, no. 2 (March 1997): 243–48. https://doi.org/10.1002/jor.1100150213.
Allen DM, Chen LE, Seaber AV, Urbaniak JR. Calcitonin gene-related peptide and reperfusion injury. J Orthop Res. 1997 Mar;15(2):243–8.
Allen, D. M., et al. “Calcitonin gene-related peptide and reperfusion injury.J Orthop Res, vol. 15, no. 2, Mar. 1997, pp. 243–48. Pubmed, doi:10.1002/jor.1100150213.
Allen DM, Chen LE, Seaber AV, Urbaniak JR. Calcitonin gene-related peptide and reperfusion injury. J Orthop Res. 1997 Mar;15(2):243–248.
Journal cover image

Published In

J Orthop Res

DOI

ISSN

0736-0266

Publication Date

March 1997

Volume

15

Issue

2

Start / End Page

243 / 248

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Time Factors
  • Reperfusion Injury
  • Rats, Sprague-Dawley
  • Rats
  • Orthopedics
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Ischemia