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Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.

Publication ,  Journal Article
Erickson, GR; Gimble, JM; Franklin, DM; Rice, HE; Awad, H; Guilak, F
Published in: Biochem Biophys Res Commun
January 18, 2002

Articular cartilage exhibits little intrinsic repair capacity, and new tissue engineering approaches are being developed to promote cartilage regeneration using cellular therapies. The goal of this study was to examine the chondrogenic potential of adipose tissue-derived stromal cells. Stromal cells were isolated from human subcutaneous adipose tissue obtained by liposuction and were expanded and grown in vitro with or without chondrogenic media in alginate culture. Adipose-derived stromal cells abundantly synthesized cartilage matrix molecules including collagen type II, VI, and chondroitin 4-sulfate. Alginate cell constructs grown in chondrogenic media for 2 weeks in vitro were then implanted subcutaneously in nude mice for 4 and 12 weeks. Immunohistochemical analysis of these samples showed significant production of cartilage matrix molecules. These findings document the ability of adipose tissue-derived stromal cells to produce characteristic cartilage matrix molecules in both in vitro and in vivo models, and suggest the potential of these cells in cartilage tissue engineering.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

January 18, 2002

Volume

290

Issue

2

Start / End Page

763 / 769

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Stromal Cells
  • Proteoglycans
  • Mice, Nude
  • Mice
  • Immunohistochemistry
  • Humans
  • Culture Techniques
  • Collagen Type VI
  • Collagen Type II
 

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Erickson, G. R., Gimble, J. M., Franklin, D. M., Rice, H. E., Awad, H., & Guilak, F. (2002). Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun, 290(2), 763–769. https://doi.org/10.1006/bbrc.2001.6270
Erickson, Geoffrey R., Jeffrey M. Gimble, Dawn M. Franklin, Henry E. Rice, Hani Awad, and Farshid Guilak. “Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.Biochem Biophys Res Commun 290, no. 2 (January 18, 2002): 763–69. https://doi.org/10.1006/bbrc.2001.6270.
Erickson GR, Gimble JM, Franklin DM, Rice HE, Awad H, Guilak F. Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun. 2002 Jan 18;290(2):763–9.
Erickson, Geoffrey R., et al. “Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.Biochem Biophys Res Commun, vol. 290, no. 2, Jan. 2002, pp. 763–69. Pubmed, doi:10.1006/bbrc.2001.6270.
Erickson GR, Gimble JM, Franklin DM, Rice HE, Awad H, Guilak F. Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun. 2002 Jan 18;290(2):763–769.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

January 18, 2002

Volume

290

Issue

2

Start / End Page

763 / 769

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Stromal Cells
  • Proteoglycans
  • Mice, Nude
  • Mice
  • Immunohistochemistry
  • Humans
  • Culture Techniques
  • Collagen Type VI
  • Collagen Type II