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Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma.

Publication ,  Journal Article
Boyle, TJ; Berend, KR; DiMaio, JM; Coles, RE; Via, DF; Lyerly, HK
Published in: Surgery
August 1993

BACKGROUND: Immunocompromised organ transplant recipients have a high incidence of B cell lymphomas (BCL). Severe combined immunodeficient (SCID) mice develop human BCL when engrafted with Epstein-Barr virus (EBV) transformed and immortalized B lymphoblastoid cell lines (BLCL). Because a lack of effective EBV-specific cytotoxic T lymphocytes (EBV-CTL) is thought to lead to lymphoma development, the SCID mouse model was used to determine the relationship between EBV-infected B cells and EBV-specific CTL in BCL development in vivo. METHODS: EBV-CTL were generated by in vitro stimulation of peripheral blood leukocytes with autologous BLCL. CD8+ CTL were isolated from CTL populations by depletion of CD4+ cells. SCID mice were engrafted with BLCL, EBV-CTL were adoptively transferred into engrafted SCID mice either immediately or 7 days after engraftment, and the animals were monitored for the development of BCL. Statistical significance was determined by the log rank test. RESULTS: SCID mice engrafted with BLCL rapidly developed BCL (mean, 20 days). SCID mice engrafted with BLCL and human leukocyte antigen-identical EBV-CTL or CD8+ EBV-CTL had a significant delay in BCL development (p < 0.05), whereas some mice did not develop BCL. In contrast, human leukocyte antigen-nonidentical EBV-CTL did not significantly delay BCL development. CONCLUSIONS: This study showed the role of EBV-CTL in inhibiting the development of BCL. A greater understanding of the cellular and viral interactions leading to B-cell transformation and malignancy may allow the development of specific interventional therapies in patients who have received immunosuppressants.

Duke Scholars

Published In

Surgery

ISSN

0039-6060

Publication Date

August 1993

Volume

114

Issue

2

Start / End Page

218 / 225

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Organ Transplantation
  • Mice, SCID
  • Mice
  • Lymphoma, B-Cell
  • Lymphocyte Activation
  • Immunotherapy, Adoptive
  • Humans
  • Herpesvirus 4, Human
 

Citation

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Boyle, T. J., Berend, K. R., DiMaio, J. M., Coles, R. E., Via, D. F., & Lyerly, H. K. (1993). Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma. Surgery, 114(2), 218–225.
Boyle, T. J., K. R. Berend, J. M. DiMaio, R. E. Coles, D. F. Via, and H. K. Lyerly. “Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma.Surgery 114, no. 2 (August 1993): 218–25.
Boyle TJ, Berend KR, DiMaio JM, Coles RE, Via DF, Lyerly HK. Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma. Surgery. 1993 Aug;114(2):218–25.
Boyle, T. J., et al. “Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma.Surgery, vol. 114, no. 2, Aug. 1993, pp. 218–25.
Boyle TJ, Berend KR, DiMaio JM, Coles RE, Via DF, Lyerly HK. Adoptive transfer of cytotoxic T lymphocytes for the treatment of transplant-associated lymphoma. Surgery. 1993 Aug;114(2):218–225.
Journal cover image

Published In

Surgery

ISSN

0039-6060

Publication Date

August 1993

Volume

114

Issue

2

Start / End Page

218 / 225

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Organ Transplantation
  • Mice, SCID
  • Mice
  • Lymphoma, B-Cell
  • Lymphocyte Activation
  • Immunotherapy, Adoptive
  • Humans
  • Herpesvirus 4, Human