Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.
Publication
, Journal Article
Maecker, HT; Moon, J; Bhatia, S; Ghanekar, SA; Maino, VC; Payne, JK; Kuus-Reichel, K; Chang, JC; Summers, A; Clay, TM; Morse, MA; Lyerly, HK ...
Published in: BMC Immunol
July 18, 2005
BACKGROUND: Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors. RESULTS: Using CMV pp65 peptide pool stimulation or pp65 HLA-A2 tetramer staining, there was significant correlation between shipped and cryopreserved samples for each assay (p
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Published In
BMC Immunol
DOI
EISSN
1471-2172
Publication Date
July 18, 2005
Volume
6
Start / End Page
17
Location
England
Related Subject Headings
- Viral Matrix Proteins
- Vaccination
- Specimen Handling
- Single-Blind Method
- Sensitivity and Specificity
- Reproducibility of Results
- Phosphoproteins
- Peptide Fragments
- Leukocytes, Mononuclear
- Laboratories
Citation
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Chicago
ICMJE
MLA
NLM
Maecker, H. T., Moon, J., Bhatia, S., Ghanekar, S. A., Maino, V. C., Payne, J. K., … Disis, M. L. (2005). Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. BMC Immunol, 6, 17. https://doi.org/10.1186/1471-2172-6-17
Maecker, Holden T., James Moon, Sonny Bhatia, Smita A. Ghanekar, Vernon C. Maino, Janice K. Payne, Kristine Kuus-Reichel, et al. “Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.” BMC Immunol 6 (July 18, 2005): 17. https://doi.org/10.1186/1471-2172-6-17.
Maecker HT, Moon J, Bhatia S, Ghanekar SA, Maino VC, Payne JK, et al. Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. BMC Immunol. 2005 Jul 18;6:17.
Maecker, Holden T., et al. “Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.” BMC Immunol, vol. 6, July 2005, p. 17. Pubmed, doi:10.1186/1471-2172-6-17.
Maecker HT, Moon J, Bhatia S, Ghanekar SA, Maino VC, Payne JK, Kuus-Reichel K, Chang JC, Summers A, Clay TM, Morse MA, Lyerly HK, DeLaRosa C, Ankerst DP, Disis ML. Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. BMC Immunol. 2005 Jul 18;6:17.
Published In
BMC Immunol
DOI
EISSN
1471-2172
Publication Date
July 18, 2005
Volume
6
Start / End Page
17
Location
England
Related Subject Headings
- Viral Matrix Proteins
- Vaccination
- Specimen Handling
- Single-Blind Method
- Sensitivity and Specificity
- Reproducibility of Results
- Phosphoproteins
- Peptide Fragments
- Leukocytes, Mononuclear
- Laboratories