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Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors.

Publication ,  Journal Article
Sullenger, BA
Published in: Appl Biochem Biotechnol
1995

The ability to target ribozymes to specifically cleave viral RNAs in vitro has led to much speculation about their potential therapeutic value as antiviral agents in vivo. To transfer a ribozyme's potential as an antiviral agent from test tubes to cells and organisms successfully, the characteristics that distinguish these settings must be considered. In vitro, ribozymes and substrate RNAs freely diffuse in solution in test tubes, and trans-cleavage reactions are dependent on a diffusive step. In eukaryotic cells, by contrast, many RNAs do not appear to diffuse freely. Instead, they appear to be highly compartmentalized and actively sorted to specific cellular locations. Such RNA trafficking may result in localization of substrate RNAs in a different compartment than ribozymes, which would effectively reduce substrate RNA availability to ribozymes and therefore limit the effectiveness of ribozymes as gene inhibitors.

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Published In

Appl Biochem Biotechnol

DOI

ISSN

0273-2289

Publication Date

1995

Volume

54

Issue

1-3

Start / End Page

57 / 61

Location

United States

Related Subject Headings

  • Substrate Specificity
  • RNA, Viral
  • RNA, Catalytic
  • Humans
  • Cell Compartmentation
  • Biotechnology
  • Animals
  • 31 Biological sciences
  • 10 Technology
  • 06 Biological Sciences
 

Citation

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Sullenger, B. A. (1995). Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors. Appl Biochem Biotechnol, 54(1–3), 57–61. https://doi.org/10.1007/BF02787911
Sullenger, B. A. “Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors.Appl Biochem Biotechnol 54, no. 1–3 (1995): 57–61. https://doi.org/10.1007/BF02787911.
Sullenger BA. Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors. Appl Biochem Biotechnol. 1995;54(1–3):57–61.
Sullenger, B. A. “Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors.Appl Biochem Biotechnol, vol. 54, no. 1–3, 1995, pp. 57–61. Pubmed, doi:10.1007/BF02787911.
Sullenger BA. Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors. Appl Biochem Biotechnol. 1995;54(1–3):57–61.
Journal cover image

Published In

Appl Biochem Biotechnol

DOI

ISSN

0273-2289

Publication Date

1995

Volume

54

Issue

1-3

Start / End Page

57 / 61

Location

United States

Related Subject Headings

  • Substrate Specificity
  • RNA, Viral
  • RNA, Catalytic
  • Humans
  • Cell Compartmentation
  • Biotechnology
  • Animals
  • 31 Biological sciences
  • 10 Technology
  • 06 Biological Sciences