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Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization.

Publication ,  Journal Article
Ibrahim, GK; Gravitt, PE; Dittrich, KL; Ibrahim, SN; Melhus, O; Anderson, SM; Robertson, CN
Published in: J Urol
December 1992

Human papillomavirus is associated with a variety of anogenital lesions, including genital warts, precancers and cancers. In male patients human papillomavirus has been identified in proliferative lesions ranging from penile and urethral warts to penile and prostatic cancers. We examined the association of human papillomavirus deoxyribonucleic acid (DNA) in 84 prostate tissue specimens. Specimens were selected from radical prostatectomy, transurethral resection or transrectal biopsy procedures. A total of 60 formalin-fixed, paraffin-embedded tissues (24 prostate cancer specimens, 16 benign prostatic hyperplasia specimens and 20 normal specimens) was examined by polymerase chain reaction and in situ hybridization. Also, 24 gelatin-embedded frozen prostate cancer specimens were examined for human papillomavirus DNA by polymerase chain reaction. Of the specimens 69 were deemed adequate for polymerase chain reaction analysis, whereas all 60 paraffin-embedded tissues were sufficient for in situ hybridization. Human papillomavirus DNA was detected in 2 normal tissues and 6 prostate cancers using polymerase chain reaction. None of the benign prostatic hyperplasia specimens was positive for human papillomavirus. Human papillomavirus typing results indicated that virus type 16 was present in each of the 8 positive specimens. Confirmation of the presence of human papillomavirus was obtained for 1 of the prostate cancers by nonisotopic in situ hybridization with biotinylated human papillomavirus genomic probes. The low prevalence of human papillomavirus in this study population does not strongly support an etiological role for the virus in prostate cancer.

Duke Scholars

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

December 1992

Volume

148

Issue

6

Start / End Page

1822 / 1826

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Polymerase Chain Reaction
  • Papillomaviridae
  • Middle Aged
  • Male
  • In Situ Hybridization
  • Humans
  • DNA Probes, HPV
 

Citation

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Ibrahim, G. K., Gravitt, P. E., Dittrich, K. L., Ibrahim, S. N., Melhus, O., Anderson, S. M., & Robertson, C. N. (1992). Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization. J Urol, 148(6), 1822–1826. https://doi.org/10.1016/s0022-5347(17)37040-4
Ibrahim, G. K., P. E. Gravitt, K. L. Dittrich, S. N. Ibrahim, O. Melhus, S. M. Anderson, and C. N. Robertson. “Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization.J Urol 148, no. 6 (December 1992): 1822–26. https://doi.org/10.1016/s0022-5347(17)37040-4.
Ibrahim GK, Gravitt PE, Dittrich KL, Ibrahim SN, Melhus O, Anderson SM, et al. Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization. J Urol. 1992 Dec;148(6):1822–6.
Ibrahim, G. K., et al. “Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization.J Urol, vol. 148, no. 6, Dec. 1992, pp. 1822–26. Pubmed, doi:10.1016/s0022-5347(17)37040-4.
Ibrahim GK, Gravitt PE, Dittrich KL, Ibrahim SN, Melhus O, Anderson SM, Robertson CN. Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization. J Urol. 1992 Dec;148(6):1822–1826.
Journal cover image

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

December 1992

Volume

148

Issue

6

Start / End Page

1822 / 1826

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Polymerase Chain Reaction
  • Papillomaviridae
  • Middle Aged
  • Male
  • In Situ Hybridization
  • Humans
  • DNA Probes, HPV