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A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy.

Publication ,  Journal Article
Toth, CA; Narayan, DG; Boppart, SA; Hee, MR; Fujimoto, JG; Birngruber, R; Cain, CP; DiCarlo, CD; Roach, WP
Published in: Arch Ophthalmol
November 1997

OBJECTIVE: To compare the cross-sectional images of primate retinal morphology obtained by optical coherence tomography (OCT) with light microscopy to determine the retinal components represented in OCT images. METHODS: Laser pulses were delivered to the retina to create small marker lesions in a Macaca mulatta. These lesions were used to align in vivo OCT scans and ex vivum histologic cross sections for image comparison. RESULTS: The OCT images demonstrated reproducible patterns of retinal morphology that corresponded to the location of retinal layers seen on light microscopic overlays. Layers of relative high reflectivity corresponded to horizontally aligned retinal components such as the nerve fiber layer and plexiform layers, as well as to the retinal pigment epithelium and choroid. In contrast, the nuclear layers and the photoreceptor inner and outer segments demonstrated relative low reflectivity by OCT. CONCLUSIONS: Retinal morphology and macular OCT imaging correlate well, with alignment of areas of high and low reflectivity to specific retinal and choroidal elements. Resolution of retinal structures by OCT depends on the contrast in relative reflectivity of adjacent structures. Use of this tool will enable expanded study of retinal morphology, both normal and pathologic, as it evolves in vivo.

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Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

November 1997

Volume

115

Issue

11

Start / End Page

1425 / 1428

Location

United States

Related Subject Headings

  • Tomography
  • Retina
  • Ophthalmology & Optometry
  • Microscopy
  • Macaca mulatta
  • Animals
  • 1113 Opthalmology and Optometry
 

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Toth, C. A., Narayan, D. G., Boppart, S. A., Hee, M. R., Fujimoto, J. G., Birngruber, R., … Roach, W. P. (1997). A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy. Arch Ophthalmol, 115(11), 1425–1428. https://doi.org/10.1001/archopht.1997.01100160595012
Toth, C. A., D. G. Narayan, S. A. Boppart, M. R. Hee, J. G. Fujimoto, R. Birngruber, C. P. Cain, C. D. DiCarlo, and W. P. Roach. “A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy.Arch Ophthalmol 115, no. 11 (November 1997): 1425–28. https://doi.org/10.1001/archopht.1997.01100160595012.
Toth CA, Narayan DG, Boppart SA, Hee MR, Fujimoto JG, Birngruber R, et al. A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy. Arch Ophthalmol. 1997 Nov;115(11):1425–8.
Toth, C. A., et al. “A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy.Arch Ophthalmol, vol. 115, no. 11, Nov. 1997, pp. 1425–28. Pubmed, doi:10.1001/archopht.1997.01100160595012.
Toth CA, Narayan DG, Boppart SA, Hee MR, Fujimoto JG, Birngruber R, Cain CP, DiCarlo CD, Roach WP. A comparison of retinal morphology viewed by optical coherence tomography and by light microscopy. Arch Ophthalmol. 1997 Nov;115(11):1425–1428.

Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

November 1997

Volume

115

Issue

11

Start / End Page

1425 / 1428

Location

United States

Related Subject Headings

  • Tomography
  • Retina
  • Ophthalmology & Optometry
  • Microscopy
  • Macaca mulatta
  • Animals
  • 1113 Opthalmology and Optometry