Skip to main content
Journal cover image

Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.

Publication ,  Journal Article
Dix, RD; Cousins, SW
Published in: Arch Virol
April 2005

Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed a rapid semiquantitative assay for infectious MCMV based on expression of beta-galactosidase using a LacZ-expressing recombinant MCMV. Simultaneous use of this assay in combination with a modified cell-mediated cytotoxicity assay revealed that cytotoxicity of MCMV-infected target cells mediated by MCMV-immune splenic cells does not lead to inactivation of intracellular infectious virus.

Duke Scholars

Published In

Arch Virol

DOI

ISSN

0304-8608

Publication Date

April 2005

Volume

150

Issue

4

Start / End Page

797 / 803

Location

Austria

Related Subject Headings

  • beta-Galactosidase
  • Virology
  • Recombinant Proteins
  • Mice
  • Cytotoxicity, Immunologic
  • Cytomegalovirus
  • Animals
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dix, R. D., & Cousins, S. W. (2005). Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus. Arch Virol, 150(4), 797–803. https://doi.org/10.1007/s00705-004-0459-8
Dix, R. D., and S. W. Cousins. “Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.Arch Virol 150, no. 4 (April 2005): 797–803. https://doi.org/10.1007/s00705-004-0459-8.
Dix, R. D., and S. W. Cousins. “Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.Arch Virol, vol. 150, no. 4, Apr. 2005, pp. 797–803. Pubmed, doi:10.1007/s00705-004-0459-8.
Journal cover image

Published In

Arch Virol

DOI

ISSN

0304-8608

Publication Date

April 2005

Volume

150

Issue

4

Start / End Page

797 / 803

Location

Austria

Related Subject Headings

  • beta-Galactosidase
  • Virology
  • Recombinant Proteins
  • Mice
  • Cytotoxicity, Immunologic
  • Cytomegalovirus
  • Animals
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences