Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.
Publication
, Journal Article
Dix, RD; Cousins, SW
Published in: Arch Virol
April 2005
Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed a rapid semiquantitative assay for infectious MCMV based on expression of beta-galactosidase using a LacZ-expressing recombinant MCMV. Simultaneous use of this assay in combination with a modified cell-mediated cytotoxicity assay revealed that cytotoxicity of MCMV-infected target cells mediated by MCMV-immune splenic cells does not lead to inactivation of intracellular infectious virus.
Duke Scholars
Published In
Arch Virol
DOI
ISSN
0304-8608
Publication Date
April 2005
Volume
150
Issue
4
Start / End Page
797 / 803
Location
Austria
Related Subject Headings
- beta-Galactosidase
- Virology
- Recombinant Proteins
- Mice
- Cytotoxicity, Immunologic
- Cytomegalovirus
- Animals
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Dix, R. D., & Cousins, S. W. (2005). Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus. Arch Virol, 150(4), 797–803. https://doi.org/10.1007/s00705-004-0459-8
Dix, R. D., and S. W. Cousins. “Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.” Arch Virol 150, no. 4 (April 2005): 797–803. https://doi.org/10.1007/s00705-004-0459-8.
Dix RD, Cousins SW. Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus. Arch Virol. 2005 Apr;150(4):797–803.
Dix, R. D., and S. W. Cousins. “Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.” Arch Virol, vol. 150, no. 4, Apr. 2005, pp. 797–803. Pubmed, doi:10.1007/s00705-004-0459-8.
Dix RD, Cousins SW. Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus. Arch Virol. 2005 Apr;150(4):797–803.
Published In
Arch Virol
DOI
ISSN
0304-8608
Publication Date
April 2005
Volume
150
Issue
4
Start / End Page
797 / 803
Location
Austria
Related Subject Headings
- beta-Galactosidase
- Virology
- Recombinant Proteins
- Mice
- Cytotoxicity, Immunologic
- Cytomegalovirus
- Animals
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences