Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.
Publication
, Journal Article
Watanabe, M; Nicchitta, CV; Blobel, G
Published in: Proc Natl Acad Sci U S A
March 1990
Proteoliposomes were reconstituted by detergent dialysis of a sodium cholate extract of inverted vesicles derived from Escherichia coli plasma membrane. The translocation of precursor proteins into reconstituted vesicles occurred at high efficiency and was SecB dependent. The protein composition of the reconstituted vesicles differed markedly from that of native vesicles. Immunoblot analysis of the sodium cholate extract and of the reconstituted vesicles indicated that PrlA (SecY) protein remained largely unsolubilized under the described conditions and was virtually absent from the reconstituted vesicles, suggesting that PrlA may not be required for in vitro translocation.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
March 1990
Volume
87
Issue
5
Start / End Page
1960 / 1964
Location
United States
Related Subject Headings
- Solubility
- SEC Translocation Channels
- RNA, Messenger
- Protein Precursors
- Protein Biosynthesis
- Monosaccharide Transport Proteins
- Membrane Proteins
- Maltose-Binding Proteins
- Maltose
- Kinetics
Citation
APA
Chicago
ICMJE
MLA
NLM
Watanabe, M., Nicchitta, C. V., & Blobel, G. (1990). Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins. Proc Natl Acad Sci U S A, 87(5), 1960–1964. https://doi.org/10.1073/pnas.87.5.1960
Watanabe, M., C. V. Nicchitta, and G. Blobel. “Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.” Proc Natl Acad Sci U S A 87, no. 5 (March 1990): 1960–64. https://doi.org/10.1073/pnas.87.5.1960.
Watanabe M, Nicchitta CV, Blobel G. Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1960–4.
Watanabe, M., et al. “Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.” Proc Natl Acad Sci U S A, vol. 87, no. 5, Mar. 1990, pp. 1960–64. Pubmed, doi:10.1073/pnas.87.5.1960.
Watanabe M, Nicchitta CV, Blobel G. Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1960–1964.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
March 1990
Volume
87
Issue
5
Start / End Page
1960 / 1964
Location
United States
Related Subject Headings
- Solubility
- SEC Translocation Channels
- RNA, Messenger
- Protein Precursors
- Protein Biosynthesis
- Monosaccharide Transport Proteins
- Membrane Proteins
- Maltose-Binding Proteins
- Maltose
- Kinetics