Skip to main content

mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation.

Publication ,  Journal Article
Lerner, RS; Nicchitta, CV
Published in: RNA
May 2006

Eukaryotic cells utilize a cycle of ribosome trafficking on the endoplasmic reticulum (ER) to partition mRNAs between the cytosol and ER compartments. In this process, ribosomes engaged in the synthesis of signal sequence-bearing proteins are trafficked to the endoplasmic reticulum via the signal-recognition particle pathway and are released from the ER upon translation termination. Though the processes governing ribosome trafficking to the ER are well understood, little is known regarding the complementary ribosome release process. In this study, Coxsackie B virus (CBV) infection was used to inactivate the initiation stage of protein synthesis, thereby limiting translation to the elongation and termination stages. Ribosome partitioning between the cytosol and ER compartments was examined to determine the role of termination in ribosome release from the ER. CBV infection resulted in efficient cleavage of eIF4G and PABP, coincident with polyribosome breakdown in the cytosol and ER compartments. Termination resulted in the continued association of ribosomes with the ER compartment, rather than the expected process of ribosome release. Analyses of ribosome/mRNA loading patterns in the cytosol and ER revealed that CBV infection was accompanied by a suppression of mRNA translation in the cytosol and the sustained, although reduced, translation in the ER compartment. Direct biosynthetic labeling experiments demonstrated that protein synthesis on the ER was enhanced relative to the cytosol following CBV infection. In total, these data demonstrate that ribosome and mRNA release from the ER is regulated independent of translation termination and identify the ER as a privileged site for protein synthesis.

Duke Scholars

Published In

RNA

DOI

ISSN

1355-8382

Publication Date

May 2006

Volume

12

Issue

5

Start / End Page

775 / 789

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Messenger
  • RNA Caps
  • Protein Synthesis Inhibitors
  • Protein Biosynthesis
  • Humans
  • Hela Cells
  • HeLa Cells
  • Eukaryotic Initiation Factor-4G
  • Enterovirus B, Human
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lerner, R. S., & Nicchitta, C. V. (2006). mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation. RNA, 12(5), 775–789. https://doi.org/10.1261/rna.2318906
Lerner, Rachel S., and Christopher V. Nicchitta. “mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation.RNA 12, no. 5 (May 2006): 775–89. https://doi.org/10.1261/rna.2318906.
Lerner, Rachel S., and Christopher V. Nicchitta. “mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation.RNA, vol. 12, no. 5, May 2006, pp. 775–89. Pubmed, doi:10.1261/rna.2318906.

Published In

RNA

DOI

ISSN

1355-8382

Publication Date

May 2006

Volume

12

Issue

5

Start / End Page

775 / 789

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Messenger
  • RNA Caps
  • Protein Synthesis Inhibitors
  • Protein Biosynthesis
  • Humans
  • Hela Cells
  • HeLa Cells
  • Eukaryotic Initiation Factor-4G
  • Enterovirus B, Human