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Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus.

Publication ,  Journal Article
Cramer, RA; Stajich, JE; Yamanaka, Y; Dietrich, FS; Steinbach, WJ; Perfect, JR
Published in: Gene
November 15, 2006

Fungi from the genus Aspergillus are important saprophytes and opportunistic human fungal pathogens that contribute in these and other diverse ways to human well-being. Part of their impact on human well-being stems from the production of small molecular weight secondary metabolites, which may contribute to the ability of these fungi to cause invasive fungal infections and allergic diseases. In this study, we identified one group of enzymes responsible for secondary metabolite production in five Aspergillus species, the non-ribosomal peptide synthetases (NRPS). Hidden Markov models were used to search the genome databases of A. fumigatus, A. flavus, A. terreus, A. nidulans, and A. oryzae for domains conserved in NRPS proteins. A genealogy of adenylation domains was utilized to identify orthologous and unique NRPS among the Aspergillus species examined, as well as gain an understanding of the potential evolution of Aspergillus NRPS. mRNA abundance of the 14 NRPS identified in the A. fumigatus genome was analyzed using real-time reverse transcriptase PCR in different environmental conditions to gain a preliminary understanding of the possible functions of the NRPSs' peptide products. Our results suggest that Aspergillus species contain conserved and unique NRPS genes with a complex evolutionary history. This result suggests that the genus Aspergillus produces a substantial diversity of non-ribosomally synthesized peptides. Further analysis of these genes and their peptide products may identify important roles for secondary metabolites produced by NRPS in Aspergillus physiology, ecology, and fungal pathogenicity.

Duke Scholars

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

November 15, 2006

Volume

383

Start / End Page

24 / 32

Location

Netherlands

Related Subject Headings

  • Sequence Homology, Amino Acid
  • RNA, Messenger
  • RNA, Fungal
  • Protein Structure, Tertiary
  • Phylogeny
  • Peptide Synthases
  • Molecular Sequence Data
  • Humans
  • Genes, Fungal
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cramer, R. A., Stajich, J. E., Yamanaka, Y., Dietrich, F. S., Steinbach, W. J., & Perfect, J. R. (2006). Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus. Gene, 383, 24–32. https://doi.org/10.1016/j.gene.2006.07.008
Cramer, Robert A., Jason E. Stajich, Yvonne Yamanaka, Fred S. Dietrich, William J. Steinbach, and John R. Perfect. “Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus.Gene 383 (November 15, 2006): 24–32. https://doi.org/10.1016/j.gene.2006.07.008.
Cramer RA, Stajich JE, Yamanaka Y, Dietrich FS, Steinbach WJ, Perfect JR. Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus. Gene. 2006 Nov 15;383:24–32.
Cramer, Robert A., et al. “Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus.Gene, vol. 383, Nov. 2006, pp. 24–32. Pubmed, doi:10.1016/j.gene.2006.07.008.
Cramer RA, Stajich JE, Yamanaka Y, Dietrich FS, Steinbach WJ, Perfect JR. Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus. Gene. 2006 Nov 15;383:24–32.
Journal cover image

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

November 15, 2006

Volume

383

Start / End Page

24 / 32

Location

Netherlands

Related Subject Headings

  • Sequence Homology, Amino Acid
  • RNA, Messenger
  • RNA, Fungal
  • Protein Structure, Tertiary
  • Phylogeny
  • Peptide Synthases
  • Molecular Sequence Data
  • Humans
  • Genes, Fungal
  • Developmental Biology