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A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.

Publication ,  Journal Article
Frech, GC; VanDongen, AM; Schuster, G; Brown, AM; Joho, RH
Published in: Nature
August 24, 1989

Voltage-activated potassium channels play an important part in the control of excitability in nerve and muscle. Different K+ channels are involved in establishing the resting potential, determining the duration of action potentials, modulation of transmitter release, and in rhythmic firing patterns and delayed excitation. Using in vitro transcripts made from a directional complementary DNA library we have isolated, by expression cloning in Xenopus oocytes, a novel K+-channel gene (drk1). Functionally, drk1 encodes channels that are K+ selective and belong to the delayed rectifier class of channels, rather than the A-type class encoded by the Shaker gene of Drosophila. The channels show sigmoidal voltage-dependent activation and do not inactivate within 500 ms. Structurally, drk1 encodes an amino-acid sequence which is more closely related to the Drosophila Shab gene than to the Shaker gene.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

August 24, 1989

Volume

340

Issue

6235

Start / End Page

642 / 645

Location

England

Related Subject Headings

  • Xenopus laevis
  • Shab Potassium Channels
  • Rats
  • Potassium Channels, Voltage-Gated
  • Potassium Channels
  • Potassium
  • Molecular Sequence Data
  • General Science & Technology
  • Electric Conductivity
  • Delayed Rectifier Potassium Channels
 

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Frech, G. C., VanDongen, A. M., Schuster, G., Brown, A. M., & Joho, R. H. (1989). A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature, 340(6235), 642–645. https://doi.org/10.1038/340642a0
Frech, G. C., A. M. VanDongen, G. Schuster, A. M. Brown, and R. H. Joho. “A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.Nature 340, no. 6235 (August 24, 1989): 642–45. https://doi.org/10.1038/340642a0.
Frech GC, VanDongen AM, Schuster G, Brown AM, Joho RH. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature. 1989 Aug 24;340(6235):642–5.
Frech, G. C., et al. “A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.Nature, vol. 340, no. 6235, Aug. 1989, pp. 642–45. Pubmed, doi:10.1038/340642a0.
Frech GC, VanDongen AM, Schuster G, Brown AM, Joho RH. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature. 1989 Aug 24;340(6235):642–645.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

August 24, 1989

Volume

340

Issue

6235

Start / End Page

642 / 645

Location

England

Related Subject Headings

  • Xenopus laevis
  • Shab Potassium Channels
  • Rats
  • Potassium Channels, Voltage-Gated
  • Potassium Channels
  • Potassium
  • Molecular Sequence Data
  • General Science & Technology
  • Electric Conductivity
  • Delayed Rectifier Potassium Channels