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Regulation of cholecystokinin secretion by bombesin in STC-1 cells.

Publication ,  Journal Article
Snow, ND; Prpic, V; Mangel, AW; Sharara, AI; McVey, DC; Hurst, LJ; Vigna, SR; Liddle, RA
Published in: Am J Physiol
November 1994

Bombesin stimulates cholecystokinin (CCK) secretion, presumably by a direct effect on the intestinal CCK cell. The present objectives were to characterize bombesin-stimulated CCK release and to investigate the role of calcium in CCK secretion in an intestinal CCK-producing cell line (STC-1). Bombesin caused a dose-dependent release of CCK, which was reduced either in the absence of extracellular calcium or by calcium channel blockade, suggesting that influx of calcium is necessary for CCK secretion. Bombesin caused an increase in intracellular calcium concentration ([Ca2+]i) and increased efflux of 45Ca2+ from 45Ca(2+)-loaded cells. Radioligand binding studies and Northern analysis were consistent with the expression of a bombesin receptor. Thus bombesin stimulation of CCK release occurs via binding to a receptor and is dependent on increased [Ca2+]i. We propose that the STC-1 cell line may provide a useful model for studying the regulation of intestinal CCK secretion.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1994

Volume

267

Issue

5 Pt 1

Start / End Page

G859 / G865

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Mice
  • Intestinal Mucosa
  • Cholecystokinin
  • Cell Line
  • Cardiovascular System & Hematology
  • Calcium
  • Bombesin
  • Animals
 

Citation

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ICMJE
MLA
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Snow, N. D., Prpic, V., Mangel, A. W., Sharara, A. I., McVey, D. C., Hurst, L. J., … Liddle, R. A. (1994). Regulation of cholecystokinin secretion by bombesin in STC-1 cells. Am J Physiol, 267(5 Pt 1), G859–G865. https://doi.org/10.1152/ajpgi.1994.267.5.G859
Snow, N. D., V. Prpic, A. W. Mangel, A. I. Sharara, D. C. McVey, L. J. Hurst, S. R. Vigna, and R. A. Liddle. “Regulation of cholecystokinin secretion by bombesin in STC-1 cells.Am J Physiol 267, no. 5 Pt 1 (November 1994): G859–65. https://doi.org/10.1152/ajpgi.1994.267.5.G859.
Snow ND, Prpic V, Mangel AW, Sharara AI, McVey DC, Hurst LJ, et al. Regulation of cholecystokinin secretion by bombesin in STC-1 cells. Am J Physiol. 1994 Nov;267(5 Pt 1):G859–65.
Snow, N. D., et al. “Regulation of cholecystokinin secretion by bombesin in STC-1 cells.Am J Physiol, vol. 267, no. 5 Pt 1, Nov. 1994, pp. G859–65. Pubmed, doi:10.1152/ajpgi.1994.267.5.G859.
Snow ND, Prpic V, Mangel AW, Sharara AI, McVey DC, Hurst LJ, Vigna SR, Liddle RA. Regulation of cholecystokinin secretion by bombesin in STC-1 cells. Am J Physiol. 1994 Nov;267(5 Pt 1):G859–G865.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1994

Volume

267

Issue

5 Pt 1

Start / End Page

G859 / G865

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Mice
  • Intestinal Mucosa
  • Cholecystokinin
  • Cell Line
  • Cardiovascular System & Hematology
  • Calcium
  • Bombesin
  • Animals