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Analyzing fibrin clot structure using a microplate reader.

Publication ,  Journal Article
Wolberg, AS; Gabriel, DA; Hoffman, M
Published in: Blood Coagul Fibrinolysis
September 2002

Fibrin clot structure studies are often performed using optical methods. For example, the clot's fiber structure can be assessed by measuring light scattering as a function of wavelength. From these measurements, one can calculate the mass/length ratio (mu), a relative measure of fibrin thickness. Fiber thickness has important functional correlates in terms of clot stability and resistance to fibrinolysis. Typically, measurements to calculate mass/length ratios are carried out on high-end spectrophotometers. However, limitations of this instrument include the large sample volume required and the inability to read multiple samples at one time. To circumvent these limitations, a plate-reading spectrophotometer is more commonly used to monitor clot formation; increases in absorbance indicate clot formation, while decreases indicate clot lysis. However, it is unclear whether plate-reading spectrophotometers can be used to quantitatively evaluate fibrin fiber structure. In the current study, we compared spectrophotometric analysis of fibrin gels on single-sample and plate-reading spectrophotometers. Results show that a plate-reading spectrophotometer does not give accurate measurements of the fiber mass/length ratio. However, the plate-reading spectrophotometer can provide a qualitative measure of fiber structure for both purified fibrinogen and plasma. We suggest that plate-reading spectrophotometers can provide a convenient, rapid, and inexpensive means of analyzing fibrin clot structure.

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Published In

Blood Coagul Fibrinolysis

DOI

ISSN

0957-5235

Publication Date

September 2002

Volume

13

Issue

6

Start / End Page

533 / 539

Location

England

Related Subject Headings

  • Thrombin
  • Spectrum Analysis
  • Reproducibility of Results
  • Kinetics
  • Humans
  • Fibrinolysis
  • Fibrin
  • Dose-Response Relationship, Drug
  • Dextrans
  • Cardiovascular System & Hematology
 

Citation

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Wolberg, A. S., Gabriel, D. A., & Hoffman, M. (2002). Analyzing fibrin clot structure using a microplate reader. Blood Coagul Fibrinolysis, 13(6), 533–539. https://doi.org/10.1097/00001721-200209000-00008
Wolberg, A. S., D. A. Gabriel, and M. Hoffman. “Analyzing fibrin clot structure using a microplate reader.Blood Coagul Fibrinolysis 13, no. 6 (September 2002): 533–39. https://doi.org/10.1097/00001721-200209000-00008.
Wolberg AS, Gabriel DA, Hoffman M. Analyzing fibrin clot structure using a microplate reader. Blood Coagul Fibrinolysis. 2002 Sep;13(6):533–9.
Wolberg, A. S., et al. “Analyzing fibrin clot structure using a microplate reader.Blood Coagul Fibrinolysis, vol. 13, no. 6, Sept. 2002, pp. 533–39. Pubmed, doi:10.1097/00001721-200209000-00008.
Wolberg AS, Gabriel DA, Hoffman M. Analyzing fibrin clot structure using a microplate reader. Blood Coagul Fibrinolysis. 2002 Sep;13(6):533–539.

Published In

Blood Coagul Fibrinolysis

DOI

ISSN

0957-5235

Publication Date

September 2002

Volume

13

Issue

6

Start / End Page

533 / 539

Location

England

Related Subject Headings

  • Thrombin
  • Spectrum Analysis
  • Reproducibility of Results
  • Kinetics
  • Humans
  • Fibrinolysis
  • Fibrin
  • Dose-Response Relationship, Drug
  • Dextrans
  • Cardiovascular System & Hematology