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Circular dichroic spectroscopy of non-human alpha-macroglobulins.

Publication ,  Journal Article
Feldman, SR; Pizzo, SV
Published in: Biochem Biophys Res Commun
September 17, 1984

Bovine, chicken and frog alpha-macroglobulins and ovomacroglobulin were studied by circular dichroic spectroscopy over the region 205-250 nm. All four spectra exhibited negative ellipticity with minima at about 215 nm similar to that reported for human alpha 2-macroglobulin. On reaction of the alpha-macroglobulins with trypsin, the spectrum of each of the four changed similarly. However, these proteins exhibited different conformational changes when treated with methylamine. These differences were exploited to determine which characteristics of alpha-macroglobulins correlate with changes in circular dichroic spectroscopy.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 17, 1984

Volume

123

Issue

2

Start / End Page

771 / 777

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Species Specificity
  • Electrophoresis, Polyacrylamide Gel
  • Circular Dichroism
  • Chickens
  • Cattle
  • Biochemistry & Molecular Biology
  • Anura
  • Animals
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Feldman, S. R., & Pizzo, S. V. (1984). Circular dichroic spectroscopy of non-human alpha-macroglobulins. Biochem Biophys Res Commun, 123(2), 771–777. https://doi.org/10.1016/0006-291x(84)90296-1
Feldman, S. R., and S. V. Pizzo. “Circular dichroic spectroscopy of non-human alpha-macroglobulins.Biochem Biophys Res Commun 123, no. 2 (September 17, 1984): 771–77. https://doi.org/10.1016/0006-291x(84)90296-1.
Feldman SR, Pizzo SV. Circular dichroic spectroscopy of non-human alpha-macroglobulins. Biochem Biophys Res Commun. 1984 Sep 17;123(2):771–7.
Feldman, S. R., and S. V. Pizzo. “Circular dichroic spectroscopy of non-human alpha-macroglobulins.Biochem Biophys Res Commun, vol. 123, no. 2, Sept. 1984, pp. 771–77. Pubmed, doi:10.1016/0006-291x(84)90296-1.
Feldman SR, Pizzo SV. Circular dichroic spectroscopy of non-human alpha-macroglobulins. Biochem Biophys Res Commun. 1984 Sep 17;123(2):771–777.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 17, 1984

Volume

123

Issue

2

Start / End Page

771 / 777

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Species Specificity
  • Electrophoresis, Polyacrylamide Gel
  • Circular Dichroism
  • Chickens
  • Cattle
  • Biochemistry & Molecular Biology
  • Anura
  • Animals
  • 3404 Medicinal and biomolecular chemistry