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The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells.

Publication ,  Journal Article
Gonzalez-Gronow, M; Kalfa, T; Johnson, CE; Gawdi, G; Pizzo, SV
Published in: J Biol Chem
July 18, 2003

Human plasminogen contains structural domains that are termed kringles. Proteolytic cleavage of plasminogen yields kringles 1-3 or 4 and kringle 5 (K5), which regulate endothelial cell proliferation. The receptor for kringles 1-3 or 4 has been identified as cell surface-associated ATP synthase; however, the receptor for K5 is not known. Sequence homology exists between the plasminogen activator streptokinase and the human voltage-dependent anion channel (VDAC); however, a functional relationship between these proteins has not been reported. A streptokinase binding site for K5 is located between residues Tyr252-Lys283, which is homologous to the primary sequence of VDAC residues Tyr224-Lys255. Antibodies against these sequences react with VDAC and detect this protein on the plasma membrane of human endothelial cells. K5 binds with high affinity (Kd of 28 nm) to endothelial cells, and binding is inhibited by these antibodies. Purified VDAC binds to K5 but only when reconstituted into liposomes. K5 also interferes with mechanisms controlling the regulation of intracellular Ca2+ via its interaction with VDAC. K5 binding to endothelial cells also induces a decrease in intracellular pH and hyperpolarization of the mitochondrial membrane. These studies suggest that VDAC is a receptor for K5.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 18, 2003

Volume

278

Issue

29

Start / End Page

27312 / 27318

Location

United States

Related Subject Headings

  • Voltage-Dependent Anion Channels
  • Streptokinase
  • Sequence Homology, Amino Acid
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Protein Binding
  • Porins
  • Plasminogen
  • Molecular Sequence Data
  • Models, Molecular
 

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Gonzalez-Gronow, M., Kalfa, T., Johnson, C. E., Gawdi, G., & Pizzo, S. V. (2003). The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells. J Biol Chem, 278(29), 27312–27318. https://doi.org/10.1074/jbc.M303172200
Gonzalez-Gronow, Mario, Theodosia Kalfa, Carrie E. Johnson, Govind Gawdi, and Salvatore V. Pizzo. “The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells.J Biol Chem 278, no. 29 (July 18, 2003): 27312–18. https://doi.org/10.1074/jbc.M303172200.
Gonzalez-Gronow M, Kalfa T, Johnson CE, Gawdi G, Pizzo SV. The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells. J Biol Chem. 2003 Jul 18;278(29):27312–8.
Gonzalez-Gronow, Mario, et al. “The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells.J Biol Chem, vol. 278, no. 29, July 2003, pp. 27312–18. Pubmed, doi:10.1074/jbc.M303172200.
Gonzalez-Gronow M, Kalfa T, Johnson CE, Gawdi G, Pizzo SV. The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells. J Biol Chem. 2003 Jul 18;278(29):27312–27318.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 18, 2003

Volume

278

Issue

29

Start / End Page

27312 / 27318

Location

United States

Related Subject Headings

  • Voltage-Dependent Anion Channels
  • Streptokinase
  • Sequence Homology, Amino Acid
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Protein Binding
  • Porins
  • Plasminogen
  • Molecular Sequence Data
  • Models, Molecular